Phase

Time

I. Peptide synthesis
1. Phosphospecific peptide synthesis (up to 15 amino acids)--10 mg used for phosphospecific epitope affinity purification, 10 mg used for antibody production, and 10 mg delivered to client
2. 10 mg non-phosphospecific peptide (up to 15 amino acids)used for affinity purification.

2 week

II. Conjugation
Conjugation of 10 mg phosphopeptide (using Cysteine) to KLH and BSA(for pep-elisa), 5mg non-phosphopeptide(for pep-elisa).

1 week

III. Immunization and anti-sera production
Eight-week protocol to produce antibodies in 2 rabbits.
Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (50 ml antiserum) will be provided to client.

8 weeks

Ⅳ.Peptide affinity purification and ELISA test.

Separating the phosphorylated antibodies from the non-phosphorylated

antibodies is performed by immuno-affinity purification, using two columns ,which does not cross-react with non-phosphospecific peptides

(confirmed by ELISA)

2 weeks

Total

13 weeks ($2500)