コントロール抗体

Control-antibodies are used to detect the specific loading control proteins.

The internal control protein is used to ensure the uniformity of protein loading, so as to determine the expression of target protein, and is an important ruler for quantitative analysis of western blotting (WB) and other experimental results. Usually, the protein selected as internal reference is stably expressed in sample cell lines or tissues with moderate expression (as far as possible, it is detected in the same linear range as the target protein). In selecting the appropriate control protein, the molecular weight of the protein (different from the target protein), subcellular distribution, and the type of experimental treatment may also be considered.

Common internal control proteins are listed in the following table. Click the name to search for corresponding antibodies:

kDa	Whole cell	Mitochondrial	Nuclear	Membrane	Cytoskeleton	Serum
125	Vinculin
110				ATPase
75						Transferrin
66			Lamin B1
60		HSP60
55			HDAC1
50	Alpha tubulin				Alpha tubulin
50	Beta tubulin				Beta tubulin
40	Beta actin				Beta actin
35	GAPDH		TBP
30		VDAC1/Porin	PCNA
25	Cyclophilin B
20	Cofilin	COX IV			Cofilin
15			Histone H3