GCKR Antibody - #AF0569
| 製品: | GCKR Antibody |
| カタログ: | AF0569 |
| タンパク質の説明: | Rabbit polyclonal antibody to GCKR |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Rabbit, Dog |
| 分子量: | 68kDa; 69kD(Calculated). |
| ユニプロット: | Q14397 |
| RRID: | AB_2834254 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0569, RRID:AB_2834254.
折りたたみ／展開
FGQTL5; GCK; GCKR; GCKR_HUMAN; GKRP; GLRE; glucokinase (hexokinase 4) regulator; Glucokinase; Glucokinase regulator; Glucokinase regulatory protein; Hexokinase 4 regulator; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; OTTHUMP00000158533;
免疫原
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
- Q14397 GCKR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14397 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K164 | Acetylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot |
研究背景
Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
Cytoplasm. Nucleus. Mitochondrion.
Note: Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus.
Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
Interacts (fructose 6-phosphate bound form) with GCK.
Fructose 1-phosphate and fructose 6-phosphate compete for the same binding site.
Belongs to the GCKR family.
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