IRF4 Antibody - #AF0692
| 製品: | IRF4 Antibody |
| カタログ: | AF0692 |
| タンパク質の説明: | Rabbit polyclonal antibody to IRF4 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Horse, Dog |
| 分子量: | 45kDa; 52kD(Calculated). |
| ユニプロット: | Q15306 |
| RRID: | AB_2834257 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0692, RRID:AB_2834257.
折りたたみ／展開
Interferon regulatory factor 4; IRF 4; IRF-4; Irf4; IRF4_HUMAN; LSIRF; Lymphocyte specific interferon regulatory factor; Lymphocyte specific IRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM 1; MUM1; NF EM5; NF-EM5; NFEM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP;
免疫原
- Q15306 IRF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15306 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K36 | Ubiquitination | Uniprot | |
| Y37 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| R201 | Methylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| Y428 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | O75116 (ROCK2) | Uniprot |
| S448 | Phosphorylation | O75116 (ROCK2) | Uniprot |
研究背景
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.
Nucleus.
Lymphoid cells.
Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with SPIB and DEF6. Directly interacts with NLRP3 in the nucleus of Th2 cells; this interaction enhances IRF4 ability to bind to the IL4 promoter and is required for optimal IRF4-dependent IL4 transcription (By similarity).
Belongs to the IRF family.
研究領域
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.