Phospho-RAD9 (Ser272) Antibody - #AF8019
| 製品: | Phospho-RAD9 (Ser272) Antibody |
| カタログ: | AF8019 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-RAD9 (Ser272) |
| アプリケーション: | WB |
| 反応性: | Human |
| 分子量: | 43kDa; 43kD(Calculated). |
| ユニプロット: | Q99638 |
| RRID: | AB_2840082 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8019, RRID:AB_2840082.
折りたたみ／展開
Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog; RAD9 homolog A; RAD9 homolog; Rad9a; RAD9A_HUMAN;
免疫原
- Q99638 RAD9A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLGSPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG
PTMs - Q99638 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Methylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | P00519 (ABL1) , A0A173G4P4 (Abl fusion) | Uniprot |
| K96 | Ubiquitination | Uniprot | |
| T167 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S277 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T292 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T313 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | P06493 (CDK1) , Q9UKI8 (TLK1) , P24941 (CDK2) | Uniprot |
| S336 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S341 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S350 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Q9UKI8-2 (TLK1) , P06493 (CDK1) | Uniprot |
| S363 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Nucleus.
Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.
Belongs to the rad9 family.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.