Phospho-PAK4/5/6 (Ser474) Antibody - #AF8027

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製品: Phospho-PAK4/5/6 (Ser474) Antibody
カタログ: AF8027
タンパク質の説明: Rabbit polyclonal antibody to Phospho-PAK4/5/6 (Ser474)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 64kd,72kDa; 64kD,81kD,75kD(Calculated).
ユニプロット: O96013 | Q9P286 | Q9NQU5
RRID: AB_2840090

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-PAK4/5/6 (Ser474) Antibody detects endogenous levels of PAK4/5/6 only when phosphorylated at Ser474.
RRID:
AB_2840090
引用形式: Affinity Biosciences Cat# AF8027, RRID:AB_2840090.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KIAA1142; p21 activated kinase 4; p21 Cdc42/Rac1-actiated kinase 4; P21 protein (Cdc42/Rac) activated kinase 4; p21(CDKN1A) activated kinase 4; p21-activated kinase 4; PAK 4; PAK-4; Pak4; PAK4_HUMAN; Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs; Serine threonine kinase PAK 4; Serine/threonine protein kinase PAK 4; Serine/threonine protein kinase PAK4; Serine/threonine-protein kinase PAK 4; EC 2.7.11.1; KIAA1264; MGC26232; p21 activated kinase 7; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A) activated kinase 7; p21-activated kinase 5; p21-activated kinase 7; PAK 5; PAK 7; PAK-5; PAK-7; PAK5; PAK7; PAK7_HUMAN; Protein kinase PAK5; Serine/threonine protein kinase PAK 7; Serine/threonine-protein kinase PAK 7; CDKN1A activated kinase 6; EC 2.7.1.37; p21 activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A) activated kinase 6; p21-activated kinase 6; p21activated kinase 6; PAK 5; PAK 6; PAK-5; PAK-6; PAK5; Pak6; PAK6_HUMAN; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; Serine/threonine-protein kinase PAK 6;

免疫原

免疫原:
Uniprot:

O96013(PAK4_HUMAN) >>Visit HPA database.

Q9P286(PAK5_HUMAN) >>Visit HPA database.

Q9NQU5(PAK6_HUMAN) >>Visit HPA database.

遺伝子(ID):
PAK6(106821730) | PAK4(56924) | PAK5(10298)
発現特異性:
O96013 PAK4_HUMAN:

Highest expression in prostate, testis and colon.

Q9P286 PAK5_HUMAN:

Predominantly expressed in brain.

Q9NQU5 PAK6_HUMAN:

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.

タンパク質配列:
MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR

MFGKKKKKIEISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTIVRGNKPCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGASSHGPGHAEENGFITFSQYSSESDTTADYTTEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDFARFSADYHSHLDSLSKPSEYSDLKWEYQRASSSSPLDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQTSPQPTMRQRSRSGSGLQEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTMCIPKVDYDRAQMVLSPPLSGSDTYPRGPAKLPQSQSKSGYSSSSHQYPSGYHKATLYHHPSLQSSSQYISTASYLSSLSLSSSTYPPPSWGSSSDQQPSRVSHEQFRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLRALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRHH

MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTVVRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPGPPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
89
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O96013/Q9P286/Q9NQU5 基板として

Site PTM Type Enzyme
Y159 Phosphorylation
K167 Acetylation
Y182 Phosphorylation
S183 Phosphorylation
S226 Phosphorylation
S270 Phosphorylation
S271 Phosphorylation
Y272 Phosphorylation
S277 Phosphorylation
T281 Phosphorylation
R285 Methylation
S286 Phosphorylation
S290 Phosphorylation
S345 Phosphorylation
S347 Phosphorylation
T349 Phosphorylation
Y350 Phosphorylation
S573 Phosphorylation Q9P286 (PAK5)
S602 Phosphorylation
T606 Phosphorylation
Site PTM Type Enzyme
K31 Ubiquitination
T33 Phosphorylation
S41 Phosphorylation
S74 Phosphorylation
K78 Methylation
S93 Phosphorylation
S97 Phosphorylation
S99 Phosphorylation Q15139 (PRKD1)
S104 Phosphorylation
R126 Methylation
S142 Phosphorylation
S148 Phosphorylation
S167 Phosphorylation
S181 Phosphorylation
T187 Phosphorylation
S195 Phosphorylation
T207 Phosphorylation
Y208 Phosphorylation
R254 Methylation
S258 Phosphorylation
S267 Phosphorylation
T277 Phosphorylation
S291 Phosphorylation
S443 Phosphorylation
S445 Phosphorylation
K455 Acetylation
K467 Acetylation
S474 Phosphorylation O96013 (PAK4)
T478 Phosphorylation
Y480 Phosphorylation
S488 Phosphorylation
K540 Ubiquitination
Site PTM Type Enzyme
T69 Phosphorylation
Y81 Phosphorylation
S83 Phosphorylation
T99 Phosphorylation
S104 Phosphorylation
S113 Phosphorylation
Y129 Phosphorylation
S132 Phosphorylation
S135 Phosphorylation
Y144 Phosphorylation
S165 Phosphorylation Q16539 (MAPK14)
S179 Phosphorylation
S207 Phosphorylation
S246 Phosphorylation
S248 Phosphorylation
S308 Phosphorylation
T326 Phosphorylation
S327 Phosphorylation
S328 Phosphorylation
T339 Phosphorylation
S346 Phosphorylation
S347 Phosphorylation
S351 Phosphorylation
T354 Phosphorylation
S360 Phosphorylation
Y365 Phosphorylation
T371 Phosphorylation
K528 Ubiquitination
S560 Phosphorylation Q9NQU5 (PAK6)
T564 Phosphorylation P52564 (MAP2K6)
Y566 Phosphorylation P52564 (MAP2K6)
S616 Phosphorylation

PTMs - O96013/Q9P286/Q9NQU5 酵素として

Substrate Site Source
P15923 (TCF3) S39 Uniprot
P15976 (GATA1) S161 Uniprot
P15976 (GATA1) S187 Uniprot
Q9P286 (PAK5) S573 Uniprot
Substrate Site Source
O96013-1 (PAK4) S99 Uniprot
O96013-1 (PAK4) S104 Uniprot
O96013-3 (PAK4) S105 Uniprot
O96013-3 (PAK4) S138 Uniprot
O96013-1 (PAK4) S148 Uniprot
O96013-1 (PAK4) S167 Uniprot
O96013-1 (PAK4) S181 Uniprot
O96013-1 (PAK4) T207 Uniprot
O96013-1 (PAK4) S258 Uniprot
O96013-1 (PAK4) S291 Uniprot
O96013-3 (PAK4) T325 Uniprot
O96013 (PAK4) S474 Uniprot
O96013-1 (PAK4) T478 Uniprot
P03372 (ESR1) S305 Uniprot
P04637 (TP53) S215 Uniprot
P18084 (ITGB5) S759 Uniprot
P18084 (ITGB5) S762 Uniprot
P35222 (CTNNB1) S675 Uniprot
P49023 (PXN) S272 Uniprot
P62826 (RAN) S135 Uniprot
Q15796 (SMAD2) S465 Uniprot
Q92934 (BAD) S75 Uniprot
Q92974-2 (ARHGEF2) S885 Uniprot
Q92974 (ARHGEF2) S886 Uniprot
Q93045 (STMN2) S50 Uniprot
Substrate Site Source
P10275 (AR) S579 Uniprot
P53667 (LIMK1) T508 Uniprot
Q00987 (MDM2) T158 Uniprot
Q00987 (MDM2) S186 Uniprot
Q92934 (BAD) S75 Uniprot
Q9BY11 (PACSIN1) S346 Uniprot
Q9NQU5 (PAK6) S560 Uniprot

研究背景

機能:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

PTMs:

Autophosphorylated on serine residues when activated by CDC42/p21 (Ref.31). Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).

Polyubiquitinated, leading to its proteasomal degradation.

細胞の位置付け:

Cytoplasm.
Note: Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest expression in prostate, testis and colon.

サブユニット構造:

Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1. Interacts with INKA1. Interacts with SH3RF2.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

機能:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

PTMs:

Autophosphorylated when activated by CDC42/p21.

細胞の位置付け:

Mitochondrion. Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in brain.

サブユニット構造:

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH.

タンパク質ファミリー:

An autoinhibitory domain is present in the N-terminal region of the protein.

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

機能:

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.

PTMs:

Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Cotranslocates into nucleus with AR in response to androgen induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.

サブユニット構造:

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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