Phospho-Histone H2B (Ser15) Antibody - #AF8033
| 製品: | Phospho-Histone H2B (Ser15) Antibody |
| カタログ: | AF8033 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Histone H2B (Ser15) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 14KD; 14kD(Calculated). |
| ユニプロット: | P33778 |
| RRID: | AB_2840096 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8033, RRID:AB_2840096.
折りたたみ／展開
H2b.1A; HIST1H2BD; HIST1H2BH; HIST1H2BL; HIST1H2BM; HIST1H2BN; HIST2H2BE; Histone H2B; Histone H2B type 1-D; Histone H2B type 1-H; Histone H2B type 1-L; Histone H2B type 1-M; Histone H2B type 1-N; Histone H2B type 2-E;
免疫原
- P33778 H2B1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P33778 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Acetylation | Uniprot | ||
| K6 | Acetylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K13 | Acetylation | Uniprot | |
| S15 | Phosphorylation | P26927 (MST1) , Q13043 (STK4) | Uniprot |
| K16 | Acetylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| K24 | Acetylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| S33 | Phosphorylation | P51812 (RPS6KA3) | Uniprot |
| K35 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K47 | Acetylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K121 | Ubiquitination | Uniprot |
研究背景
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Nucleus. Chromosome.
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Belongs to the histone H2B family.
研究領域
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
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