Phospho-MAX (Ser11) Antibody - #AF8034
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8034, RRID:AB_2840097.
折りたたみ／展開
bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; Helix loop helix zipper protein; MAX protein; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; MYC associated factor X; Myc binding novel HLH/LZ protein; Orf 1; Orf1; Protein max;
免疫原
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.
- P61244 MAX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P61244 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S11 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K24 | Ubiquitination | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K66 | Acetylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| R75 | Methylation | Uniprot | |
| K89 | Methylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K153 | Acetylation | Uniprot | |
| K154 | Acetylation | Uniprot |
研究背景
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.
Nucleus. Cell projection>Dendrite.
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity.
Belongs to the MAX family.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
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