Phospho-FES (Tyr713) Antibody - #AF8041
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8041, RRID:AB_2840104.
折りたたみ／展開
c-fes/fps protein; cFES; Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog; feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami; Feline sarcoma oncogene; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; fes; FES proto oncogene, tyrosine kinase; FES_HUMAN; FPS; Oncogene FES; Oncogene FES feline sarcoma virus; P93c fes; Proto oncogene tyrosine protein kinase Fes/Fps; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; Tyrosine-protein kinase Fes/Fps;
免疫原
Widely expressed. Detected in adult colon epithelium (at protein level) (PubMed:16455651, PubMed:19051325). Expressed in melanocytes (at protein level) (PubMed:28463229).
- P07332 FES_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSKLSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPVLLLQDDRHSTSSSEQEREGGRTPTLEILKSHISGIFRPKFSLPPPLQLIPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLWDGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRAVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLPPDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTFLRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVLKISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCWAYEPGQRPSFSTIYQELQSIRKRHR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P07332 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| Y461 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| T595 | Phosphorylation | Uniprot | |
| Y614 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| Y713 | Phosphorylation | P07332 (FES) | Uniprot |
| S716 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| Y734 | Phosphorylation | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| Y811 | Phosphorylation | P07332 (FES) | Uniprot |
PTMs - P07332 酵素として
研究背景
Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down-stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28.
Autophosphorylated on Tyr-713. Phosphorylated by LYN in response to FCER1 activation. Phosphorylated by HCK.
Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle. Golgi apparatus. Cell junction>Focal adhesion.
Note: Distributed throughout the cytosol when the kinase is not activated. Association with microtubules requires activation of the kinase activity. Shuttles between focal adhesions and cell-cell contacts in epithelial cells. Recruited to the lateral cell membrane in polarized epithelial cells by interaction with phosphorylated EZR. Detected at tubular membrane structures in the cytoplasm and at the cell periphery.
Widely expressed. Detected in adult colon epithelium (at protein level). Expressed in melanocytes (at protein level).
Homooligomer. Interacts with BCR. Interacts (when activated, via coiled coil domain) with TRIM28. Interacts (via SH2 domain) with phosphorylated EZR, MS4A2/FCER1B and HCLS1/HS1. Interacts with phosphorylated KIT. Interacts with FLT3. Interacts (via F-BAR domain) with soluble tubulin. Interacts (via SH2 domain) with microtubules.
The coiled coil domains are important for regulating the kinase activity. They mediate homooligomerization and probably also interaction with other proteins.
The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
研究領域
· Organismal Systems > Development > Axon guidance. (View pathway)
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