製品: KSR2 Antibody
カタログ: AF0688
タンパク質の説明: Rabbit polyclonal antibody to KSR2
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 108kDa; 108kD(Calculated).
ユニプロット: Q6VAB6
RRID: AB_2834291

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(91%), Zebrafish(91%), Bovine(100%), Horse(91%), Sheep(91%), Rabbit(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
KSR2 Antibody detects endogenous levels of total KSR2.
RRID:
AB_2834291
引用形式: Affinity Biosciences Cat# AF0688, RRID:AB_2834291.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FLJ25965; hKSR 2; hKSR2; Kinase suppressor of Ras 2; KSR 2; Ksr2; KSR2_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q6VAB6 KSR2_HUMAN:

Mainly expressed in brain and kidney.

タンパク質の説明:
KSR2 Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Interacts with MAP2K1, MAP3K8, MAPK, RAS and RAF. 2 isoforms of the human protein are produced by alternative splicing.
タンパク質配列:
MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Xenopus
100
Chicken
100
Rabbit
100
Pig
91
Horse
91
Sheep
91
Zebrafish
91
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6VAB6 基板として

Site PTM Type Enzyme
S34 Phosphorylation
K44 Ubiquitination
T260 Phosphorylation
T264 Phosphorylation
T269 Phosphorylation
T270 Phosphorylation
T272 Phosphorylation
T276 Phosphorylation
K281 Acetylation
T290 Phosphorylation
S294 Phosphorylation
T306 Phosphorylation
S311 Phosphorylation
S313 Phosphorylation
S347 Phosphorylation
S394 Phosphorylation
S399 Phosphorylation
S474 Phosphorylation
S490 Phosphorylation
T497 Phosphorylation
Y566 Phosphorylation
Y794 Phosphorylation
T802 Phosphorylation
S808 Phosphorylation
K923 Ubiquitination
S939 Phosphorylation

PTMs - Q6VAB6 酵素として

Substrate Site Source
Q02750 (MAP2K1) S24 Uniprot
Q02750 (MAP2K1) S72 Uniprot

研究背景

機能:

Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

PTMs:

Phosphorylated on Ser-474 by MARK3.

細胞の位置付け:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in brain and kidney.

サブユニット構造:

Heterodimerizes (via N-terminus) with BRAF (via N-terminus) in a MAP2K1/MEK1-dependent manner. Interacts with BRAF; this increases the low intrinsic protein kinase activity of KSR2. Interacts with MAP2K1, forming a heterodimer that can dimerize to form a heterotetramer. Interacts with MAP3K8, MAPK, RAS and RAF.

タンパク質ファミリー:

The protein kinase domain is predicted to be catalytically inactive and seems to have very low intrinsic kinase activity. This low kinase activity can be increased by interaction with BRAF.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

研究領域

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

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