DOK3 Antibody - #AF0600

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製品: DOK3 Antibody
カタログ: AF0600
タンパク質の説明: Rabbit polyclonal antibody to DOK3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Rabbit, Dog
分子量: 58kDa; 53kD(Calculated).
ユニプロット: Q7L591
RRID: AB_2834292

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
DOK3 Antibody detects endogenous levels of total DOK3.
RRID:
AB_2834292
引用形式: Affinity Biosciences Cat# AF0600, RRID:AB_2834292.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Docking protein 3; DOK 3; DOK Like Protein; Dok3; DOK3_HUMAN; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein;

免疫原

免疫原:
Uniprot:

Q7L591(DOK3_HUMAN) >>Visit HPA database.

遺伝子(ID):
DOK3(79930)
発現特異性:
Q7L591 DOK3_HUMAN:

Expressed in spleen.

タンパク質の説明:
Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP. May modulate Abl function. On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP. 4 isoforms of the human protein are produced by alternative splicing.
タンパク質配列:
MTRGARLRSDARAQLNQLSLDGGTGSGQKGKCEEFPSSLSSVSPGLEAAALLLAVTMDPLETPIKDGILYQQHVKFGKKCWRKVWALLYAGGPSGVARLESWEVRDGGLGAAGDRSAGPGRRGERRVIRLADCVSVLPADGESCPRDTGAFLLTTTERSHLLAAQHRQAWMGPICQLAFPGTGEASSGSTDAQSPKRGLVPMEENSIYSSWQEVGEFPVVVQRTEAATRCQLKGPALLVLGPDAIQLREAKGTQALYSWPYHFLRKFGSDKGVFSFEAGRRCHSGEGLFAFSTPCAPDLCRAVAGAIARQRERLPELTRPQPCPLPRATSLPSLDTPGELREMPPGPEPPTSRKMHLAEPGPQSLPLLLGPEPNDLASGLYASVCKRASGPPGNEHLYENLCVLEASPTLHGGEPEPHEGPGSRSPTTSPIYHNGQDLSWPGPANDSTLEAQYRRLLELDQVEGTGRPDPQAGFKAKLVTLLSRERRKGPAPCDRP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Chicken
70
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L591 基板として

Site PTM Type Enzyme
S9 Phosphorylation
Y89 Phosphorylation
R122 Methylation
S194 Phosphorylation
Y208 Phosphorylation
T329 Phosphorylation
S330 Phosphorylation
S333 Phosphorylation
T336 Phosphorylation
T351 Phosphorylation
S352 Phosphorylation
S364 Phosphorylation
S378 Phosphorylation
Y381 Phosphorylation
S389 Phosphorylation
Y398 Phosphorylation
S407 Phosphorylation
Y432 Phosphorylation
Y453 Phosphorylation
K477 Acetylation
T480 Phosphorylation
S483 Phosphorylation

研究背景

機能:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity).

PTMs:

Constitutively tyrosine-phosphorylated.

On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).

細胞の位置付け:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in spleen.

サブユニット構造:

On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL1 through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP (By similarity).

タンパク質ファミリー:

PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

参考文献

1). Dok3 is involved in cisplatin-induced acute kidney injury via regulation of inflammation and apoptosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34245977) [IF=3.1]

Application: IHC    Species: Mouse    Sample: Kidney tissues

Fig.1. Deletion of Dok3 attenuates cisplatin-induced AKI. (A) Level of BUN in the serum. (A) Level of creatinine in the serum; (C) Images of immunohistochemical staining of NGAL; 400 ? magnification. (D) Quantification of immunohistochemical staining of NGAL. (E) Analysis of survival rate of mice. All values are expressed as the mean ± standard error. n ¼ 6, **P < 0.01, and ***P < 0.001.

Application: WB    Species: Mouse    Sample: Kidney tissues

Fig. 4. Overexpression of DOK3 exacerbates apoptosis and inflammation in vitro. (A) Dok3 mRNA expression in mouse kidney tissues. (B) Dok3 protein levels in mouse kidney tissues. (C) Images of immunohistochemical (IHC) staining of DOK3 (400 ? magnification). (D) Images of immunofluorescence staining of DOK3/AQP1 (400 ? magnification). (E) Dok3 protein levels in HK2 cells. (F) Western blot analysis verifying pcDNA-Dok3 expression. (G) PI-Hochest staining of pcDNA-Dok3-transfected HK2 cells with/without cisplatin treatment. (H) LDH level in the cell culture supernatant. (I) TNF- a level in cell culture supernatant. (J) IL-6 level in cell culture supernatant. All values are expressed as the mean ± standard error, *P < 0.05, **P < 0.01, and ***P < 0.001.

Application: IF/ICC    Species: Mouse    Sample: Kidney tissues

Fig. 4. Overexpression of DOK3 exacerbates apoptosis and inflammation in vitro. (A) Dok3 mRNA expression in mouse kidney tissues. (B) Dok3 protein levels in mouse kidney tissues. (C) Images of immunohistochemical (IHC) staining of DOK3 (400 ? magnification). (D) Images of immunofluorescence staining of DOK3/AQP1 (400 ? magnification). (E) Dok3 protein levels in HK2 cells. (F) Western blot analysis verifying pcDNA-Dok3 expression. (G) PI-Hochest staining of pcDNA-Dok3-transfected HK2 cells with/without cisplatin treatment. (H) LDH level in the cell culture supernatant. (I) TNF- a level in cell culture supernatant. (J) IL-6 level in cell culture supernatant. All values are expressed as the mean ± standard error, *P < 0.05, **P < 0.01, and ***P < 0.001.

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