Phospho-Syndecan 4 (Ser179) Antibody - #AF8061

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製品: Phospho-Syndecan 4 (Ser179) Antibody
カタログ: AF8061
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Syndecan 4 (Ser179)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 22kDa; 22kD(Calculated).
ユニプロット: P31431
RRID: AB_2840124

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(89%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-Syndecan 4 (Ser179) Antibody detects endogenous levels of Syndecan 4 only when phosphorylated at Ser179.
RRID:
AB_2840124
引用形式: Affinity Biosciences Cat# AF8061, RRID:AB_2840124.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;

免疫原

免疫原:
Uniprot:

P31431(SDC4_HUMAN) >>Visit HPA database.

遺伝子(ID):
SDC4(6385)
発現特異性:
P31431 SDC4_HUMAN:

Expressed in epithelial and fibroblastic cells.

タンパク質配列:
MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Dog
89
Rabbit
89
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31431 基板として

Site PTM Type Enzyme
S97 O-Glycosylation
T101 O-Glycosylation
K104 Ubiquitination
S117 O-Glycosylation
S126 O-Glycosylation
T134 Phosphorylation
K174 Ubiquitination
S179 Phosphorylation Q05655 (PRKCD)
Y180 Phosphorylation P12931 (SRC)
K190 Ubiquitination
T193 Phosphorylation
Y197 Phosphorylation

研究背景

機能:

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

PTMs:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

細胞の位置付け:

Membrane>Single-pass type I membrane protein. Secreted.
Note: Shedding of the ectodomain produces a soluble form.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in epithelial and fibroblastic cells.

サブユニット構造:

Homodimer. Interacts (via its cytoplasmic domain) with GIPC (via its PDZ domain). Interacts (via its cytoplasmic domain) with NUDT16L1 (By similarity). Interacts with CDCP1 and SDCBP.

タンパク質ファミリー:

Belongs to the syndecan proteoglycan family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

参考文献

1). Phosphorylation-regulated phase separation of syndecan-4 and syntenin promotes the biogenesis of exosomes. Cell proliferation, 2024 (PubMed: 38601993) [IF=8.5]

Application: WB    Species: Human    Sample: HeLa cells

FIGURE 3 Dephosphorylation of SDC4 increased SDC4 phase sepration and the recruitment of syntenin on the plasma membrane (PM). (A) Confocal laser scanning microscopy (CLSM) images of Staurosporine and PMA regulate SDC4 clusters on the PM of HeLa cells. An anti-SDC4-ecto antibody and a Cy3-labelled secondary antibody were used to detect the location of SDC4. (B) SDC4-eGFP-syntenin cells were stimulated with PKC-activating phorbol ester (PMA) and staurosporine. Dimethyl sulfoxide (DMSO) was used as the control. (C) For the quantification of the subcellular localization of syntenin stimulated with PMA or staurosporine, the number of cells that have syntenin in the cytoplasm and at the plasma membrane was analysed. The y-axis represents the ratio of the number of cells that have syntenin on the membrane to those in the cytoplasm. The results were calculated in 3 independent experiments (**p 

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