Phospho-Integrin alpha4 (Ser1027) Antibody - #AF8071
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8071, RRID:AB_2840134.
折りたたみ／展開
269C wild type; Antigen CD49D, alpha 4 subunit of VLA 4 receptor; CD49 antigen like family member D; CD49 antigen-like family member D; CD49d; IA4; Integrin alpha 4; Integrin alpha 4 subunit; Integrin alpha IV; Integrin alpha-4; Integrin alpha-IV; Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor); ITA4_HUMAN; ITGA4; MGC90518; OTTHUMP00000205320; Very late activation protein 4 receptor, alpha 4 subunit; VLA 4 subunit alpha; VLA-4 subunit alpha; VLA4;
免疫原
- P13612 ITA4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAWEARREPGPRRAAVRETVMLLLCLGVPTGRPYNVDTESALLYQGPHNTLFGYSVVLHSHGANRWLLVGAPTANWLANASVINPGAIYRCRIGKNPGQTCEQLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGSIVTCGHRWKNIFYIKNENKLPTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAGISSFYTKDLIVMGAPGSSYWTGSLFVYNITTNKYKAFLDKQNQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHEQIGKAYIFSIDEKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGSGAVMNAMETNLVGSDKYAARFGESIVNLGDIDNDGFEDVAIGAPQEDDLQGAIYIYNGRADGISSTFSQRIEGLQISKSLSMFGQSISGQIDADNNGYVDVAVGAFRSDSAVLLRTRPVVIVDASLSHPESVNRTKFDCVENGWPSVCIDLTLCFSYKGKEVPGYIVLFYNMSLDVNRKAESPPRFYFSSNGTSDVITGSIQVSSREANCRTHQAFMRKDVRDILTPIQIEAAYHLGPHVISKRSTEEFPPLQPILQQKKEKDIMKKTINFARFCAHENCSADLQVSAKIGFLKPHENKTYLAVGSMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P13612 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K95 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| N480 | N-Glycosylation | Uniprot | |
| N518 | N-Glycosylation | Uniprot | |
| N538 | N-Glycosylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| S634 | Phosphorylation | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| N645 | N-Glycosylation | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| K771 | Ubiquitination | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| K906 | Ubiquitination | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| K1010 | Ubiquitination | Uniprot | |
| S1011 | Phosphorylation | Uniprot | |
| S1021 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1023 | Phosphorylation | Uniprot | |
| Y1024 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| K1028 | Ubiquitination | Uniprot | |
| S1029 | Phosphorylation | Uniprot |
研究背景
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Phosphorylation on Ser-1027 inhibits PXN binding.
Membrane>Single-pass type I membrane protein.
Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with JAML; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling JAML homodimerization. ITGA4:ITGB1 is found in a ternary complex with CX3CR1 and CX3CL1. Interacts with MDK. ITGA4:ITGB1 interacts with MDK; this interaction mediates MDK-induced osteoblast cells migration through PXN phosphorylation.
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
Belongs to the integrin alpha chain family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Immune system > Intestinal immune network for IgA production. (View pathway)
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