Phospho-Centaurin beta-1 (Ser554) Antibody - #AF8083
| 製品: | Phospho-Centaurin beta-1 (Ser554) Antibody |
| カタログ: | AF8083 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Centaurin beta-1 (Ser554) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 分子量: | 81KD; 82kD(Calculated). |
| ユニプロット: | Q15027 |
| RRID: | AB_2840146 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8083, RRID:AB_2840146.
折りたたみ／展開
ACAP 1; ACAP1; ACAP1_HUMAN; Arf GAP with coiled coil ANK repeat and PH domains 1; Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; ArfGAP with coiled-coil, ankyrin repeat and PH domains 1; CENT B1; Centaurin beta 1; Centaurin-beta-1; CENTB 1; Cnt b1; Cnt-b1; Cntb1; KIAA0050;
免疫原
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
- Q15027 ACAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL
PTMs - Q15027 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Acetylation | Uniprot | |
| K4 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K558 | Ubiquitination | Uniprot | |
| T711 | Phosphorylation | Uniprot | |
| Y712 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| T739 | Phosphorylation | Uniprot |
研究背景
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.
PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers.
The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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