Phospho-STAT4 (Ser721) Antibody - #AF8128
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8128, RRID:AB_2840191.
折りたたみ／展開
Signal transducer and activator of transcription 4; SLEB11; STAT4; STAT4_HUMAN;
免疫原
- Q14765 STAT4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14765 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S130 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| T284 | Phosphorylation | Uniprot | |
| T287 | Phosphorylation | Uniprot | |
| Y288 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| Y645 | Phosphorylation | Uniprot | |
| Y693 | Phosphorylation | O60674 (JAK2) | Uniprot |
| S713 | Phosphorylation | Uniprot | |
| S721 | Phosphorylation | Q16539 (MAPK14) , P52564 (MAP2K6) | Uniprot |
| S733 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| Y745 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot |
研究背景
Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).
Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.
Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP (By similarity). The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain.
Belongs to the transcription factor STAT family.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
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