Phospho-BLK (Tyr389) Antibody - #AF8133
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8133, RRID:AB_2840196.
折りたたみ／展開
B lymphocyte kinase; B lymphoid tyrosine kinase; Blk; BLK nonreceptor tyrosine kinase; BLK_HUMAN; EC 2.7.10.2; MGC 10442; MGC10442; MODY 11; MODY11; P55 BLK; p55-BLK; Tyrosine kinase B lymphocyte specific; Tyrosine protein kinase; Tyrosine-protein kinase BLK;
免疫原
Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
- P51451 BLK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51451 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| Y187 | Phosphorylation | Uniprot | |
| Y188 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| Y314 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| Y350 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| Y494 | Phosphorylation | Uniprot | |
| Y501 | Phosphorylation | Uniprot |
PTMs - P51451 酵素として
| Substrate | Site | Source |
|---|---|---|
| P11912 (CD79A) | Y188 | Uniprot |
| P11912 (CD79A) | Y199 | Uniprot |
| P12318-2 (FCGR2A) | Y280 | Uniprot |
| P12318 (FCGR2A) | Y281 | Uniprot |
| P12318-2 (FCGR2A) | Y287 | Uniprot |
| P12318 (FCGR2A) | Y288 | Uniprot |
| P12318-2 (FCGR2A) | Y303 | Uniprot |
| P12318 (FCGR2A) | Y304 | Uniprot |
| P31994-5 (FCGR2B) | Y272 | Uniprot |
| P31994-2 (FCGR2B) | Y273 | Uniprot |
| P31994-4 (FCGR2B) | Y291 | Uniprot |
| P31994 (FCGR2B) | Y292 | Uniprot |
| P31995 (FCGR2C) | Y294 | Uniprot |
| P31995 (FCGR2C) | Y310 | Uniprot |
| P40259 (CD79B) | Y196 | Uniprot |
| P40259 (CD79B) | Y207 | Uniprot |
研究背景
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling (By similarity). B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors (By similarity). Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (By similarity). Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor (By similarity). Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr-207' (By similarity). Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation (By similarity). Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation (By similarity). In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Phosphorylates CGAS, promoting retention of CGAS in the cytosol.
Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR).
Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.
Cell membrane>Lipid-anchor.
Note: Present and active in lipid rafts. Membrane location is required for the phosphorylation of CD79A and CD79B (By similarity).
Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
Interacts with CBL (via SH2 domain). Interacts with CD79A and CD79B (via SH2 domain) (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
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