Phospho-BRK (Tyr342) Antibody - #AF8134
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8134, RRID:AB_2840197.
折りたたみ/展開
Breast tumor kinase; Breast tumour kinase; Protein tyrosine kinase 6; Protein-tyrosine kinase 6; Ptk6; PTK6_HUMAN; Tyrosine protein kinase BRK; Tyrosine-protein kinase BRK;
免疫原
Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines.
- Q13882 PTK6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLDEAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGAFLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13882 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y13 | Phosphorylation | Q13882 (PTK6) | Uniprot |
Y61 | Phosphorylation | Q13882 (PTK6) | Uniprot |
Y66 | Phosphorylation | Q13882 (PTK6) | Uniprot |
S111 | Phosphorylation | Uniprot | |
Y114 | Phosphorylation | Q13882 (PTK6) | Uniprot |
S117 | Phosphorylation | Uniprot | |
Y127 | Phosphorylation | Uniprot | |
S159 | Phosphorylation | Uniprot | |
S279 | Phosphorylation | Uniprot | |
Y342 | Phosphorylation | Q13882 (PTK6) | Uniprot |
S344 | Phosphorylation | Uniprot | |
Y351 | Phosphorylation | Q13882 (PTK6) | Uniprot |
K352 | Ubiquitination | Uniprot | |
T445 | Phosphorylation | Uniprot | |
S446 | Phosphorylation | Uniprot | |
Y447 | Phosphorylation | Q13882 (PTK6) , Q9H3Y6 (SRMS) | Uniprot |
PTMs - Q13882 酵素として
Substrate | Site | Source |
---|---|---|
P00533 (EGFR) | Y869 | Uniprot |
P31749 (AKT1) | Y315 | Uniprot |
P31749 (AKT1) | Y326 | Uniprot |
P35222 (CTNNB1) | Y64 | Uniprot |
P35222 (CTNNB1) | Y142 | Uniprot |
P35222 (CTNNB1) | Y331 | Uniprot |
P35222 (CTNNB1) | Y333 | Uniprot |
P40763 (STAT3) | Y705 | Uniprot |
P42229 (STAT5A) | Y694 | Uniprot |
P49023-2 (PXN) | Y31 | Uniprot |
P49023-2 (PXN) | Y118 | Uniprot |
P51692 (STAT5B) | Y699 | Uniprot |
P56945 (BCAR1) | Y165 | Uniprot |
P56945 (BCAR1) | Y224 | Uniprot |
P56945 (BCAR1) | Y234 | Uniprot |
P56945 (BCAR1) | Y249 | Uniprot |
P56945 (BCAR1) | Y262 | Uniprot |
P56945 (BCAR1) | Y267 | Uniprot |
P56945 (BCAR1) | Y287 | Uniprot |
P56945 (BCAR1) | Y306 | Uniprot |
P56945 (BCAR1) | Y327 | Uniprot |
P56945 (BCAR1) | Y362 | Uniprot |
P56945 (BCAR1) | Y387 | Uniprot |
P56945 (BCAR1) | Y664 | Uniprot |
Q05397 (PTK2) | Y861 | Uniprot |
Q07666 (KHDRBS1) | Y435 | Uniprot |
Q07666 (KHDRBS1) | Y440 | Uniprot |
Q07666-1 (KHDRBS1) | Y443 | Uniprot |
Q13017 (ARHGAP5) | Y1109 | Uniprot |
Q13882 (PTK6) | Y13 | Uniprot |
Q13882 (PTK6) | Y61 | Uniprot |
Q13882 (PTK6) | Y66 | Uniprot |
Q13882 (PTK6) | Y114 | Uniprot |
Q13882-1 (PTK6) | Y342 | Uniprot |
Q13882 (PTK6) | Y351 | Uniprot |
Q13882 (PTK6) | Y447 | Uniprot |
Q96P48 (ARAP1) | Y231 | Uniprot |
Q99704 (DOK1) | Y362 | Uniprot |
Q9NRY4 (ARHGAP35) | Y1105 | Uniprot |
Q9UGK3 (STAP2) | Y250 | Uniprot |
研究背景
Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.
Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.
Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity.
Cytoplasm. Nucleus. Cell projection>Ruffle. Membrane.
Note: Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. Nuclear localization in epithelial cells of normal prostate but cytoplasmic localization in cancer prostate.
Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines.
Interacts with GAP-A.p65 (By similarity). Interacts (via SH3 and SH2 domains) with KHDRBS1. Interacts (via SH3 and SH2 domains) with phosphorylated IRS4. Interacts with ADAM15. Interacts (via SH3 domain) with SFPQ. Interacts with EGFR and ERBB2. Interacts with STAP2. Interacts with PNX. Interacts with SFPQ. Interacts with PTK/ATK. Interacts with CTNNB1.
The SH3 domain plays a major role in substrate interactions. The SH2 domain of PTK6 plays a role in protein-protein interactions, but is likely more important for the regulation of catalytic activity.
Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
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