Phospho-MAP3K2 (Ser520) Antibody - #AF8166
| 製品: | Phospho-MAP3K2 (Ser520) Antibody |
| カタログ: | AF8166 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-MAP3K2 (Ser520) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 70kDa; 70kD(Calculated). |
| ユニプロット: | Q9Y2U5 |
| RRID: | AB_2840228 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8166, RRID:AB_2840228.
折りたたみ／展開
M3K2_HUMAN; Map3k2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2b; Mitogen activated protein kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2;
免疫原
- Q9Y2U5 M3K2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y2U5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S26 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| Y250 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K260 | Methylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| T283 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| T516 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| T522 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K602 | Ubiquitination | Uniprot |
研究背景
Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
Autophosphorylated.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.
Cytoplasm. Nucleus.
Note: Upon EGF stimulation, translocates into the nucleus.
Interacts with PKN2; the interaction activates PKN2 kinase activity in a MAP3K2-independent kinase activity (By similarity). Self-associates. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts (via the kinase catalytic domain) with STK38. Interacts with XIAP/BIRC4.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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