Phospho-MST3 (Thr18) Antibody - #AF8173
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8173, RRID:AB_2840235.
折りたたみ／展開
epididymis secretory protein Li 95; HEL-S-95; Mammalian STE20 like protein kinase 3; Mammalian STE20-like protein kinase 3; Mammalian STE20-like protein kinase 3 C-terminal; Mammalian STE20-like protein kinase 3 N-terminal; Mammalian sterile 20-like 3; MST-3; MST3/C; MST3/N; MST3B; serine threonine kinase 24; Serine/threonine kinase 24 (Ste20, yeast homolog); Serine/threonine-protein kinase 24 12 kDa subunit; Serine/threonine-protein kinase 24; STE20 homolog yeast; STE20 like kinase MST3; STE20-like kinase MST3; Stk24; STK24_HUMAN; STK3;
免疫原
Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
- Q9Y6E0 STK24_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSRAQLWGLALNKRRATLPHPGGSTNLKADPEELFTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKEFVEACLNKEPSFRPTAKELLKHKFILRNAKKTSYLTELIDRYKRWKAEQSHDDSSSEDSDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6E0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T18 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K38 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T190 | Phosphorylation | Q9Y6E0 (STK24) | Uniprot |
| T194 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| Y433 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot |
PTMs - Q9Y6E0 酵素として
研究背景
Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.
Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.
Cytoplasm. Nucleus. Membrane.
Note: The truncated form (MST3/N) translocates to the nucleus. Colocalizes with STK38L in the membrane.
Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
Monomer. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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