Phospho-NDR1/2 (Thr444/Thr442) Antibody - #AF8174
| 製品: | Phospho-NDR1/2 (Thr444/Thr442) Antibody |
| カタログ: | AF8174 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-NDR1/2 (Thr444/Thr442) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 54KD; 54kD(Calculated). |
| ユニプロット: | Q15208 | Q9Y2H1 |
| RRID: | AB_2840236 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8174, RRID:AB_2840236.
折りたたみ／展開
NDR 1; NDR; Ndr Ser/Thr kinase-like protein; NDR1; NDR1 protein kinase; Nuclear Dbf2 related kinase 1; nuclear Dbf2-related 1; Nuclear Dbf2-related kinase 1; Nuclear Dbf2-related protein; Serine/threonine protein kinase 38; Serine/threonine-protein kinase 38; STK 38; STK38; STK38_HUMAN; KIAA0965; NDR2; NDR2 protein kinase; nuclear Dbf2 related 2; Nuclear Dbf2 related kinase 2; Nuclear Dbf2-related kinase 2; Serine/threonine protein kinase 38 like; Serine/threonine-protein kinase 38-like; ST38L_HUMAN; Stk38l;
免疫原
Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Q9Y2H1 ST38L_HUMAN:Ubiquitously expressed with highest levels observed in the thymus.
- Q15208 STK38_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMTGSTPCSSMSNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPISEKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK
- Q9Y2H1 ST38L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMTAGTTTTFPMSNHTRERVTVAKLTLENFYSNLILQHEERETRQKKLEVAMEEEGLADEEKKLRRSQHARKETEFLRLKRTRLGLDDFESLKVIGRGAFGEVRLVQKKDTGHIYAMKILRKSDMLEKEQVAHIRAERDILVEADGAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYISETVLAIDAIHQLGFIHRDIKPDNLLLDAKGHVKLSDFGLCTGLKKAHRTEFYRNLTHNPPSDFSFQNMNSKRKAETWKKNRRQLAYSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKVMNWKETLVFPPEVPISEKAKDLILRFCIDSENRIGNSGVEEIKGHPFFEGVDWEHIRERPAAIPIEIKSIDDTSNFDDFPESDILQPVPNTTEPDYKSKDWVFLNYTYKRFEGLTQRGSIPTYMKAGKL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15208/Q9Y2H1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| T7 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S10 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S11 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S13 | Phosphorylation | Uniprot | |
| T16 | Phosphorylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| K58 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| T74 | Phosphorylation | Q15208 (STK38) | Uniprot |
| K93 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| C234 | S-Nitrosylation | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Q15208 (STK38) | Uniprot |
| T282 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| Y288 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| T407 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| Y443 | Phosphorylation | Uniprot | |
| T444 | Phosphorylation | P26927 (MST1) , Q15208 (STK38) , Q9Y6E0 (STK24) , Q13043 (STK4) | Uniprot |
| Y445 | Phosphorylation | Uniprot | |
| T452 | Phosphorylation | Uniprot | |
| R454 | Methylation | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K73 | Ubiquitination | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S282 | Phosphorylation | Q9Y2H1 (STK38L) | Uniprot |
| T283 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| Y289 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| Y441 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Q9Y6E0 (STK24) | Uniprot |
| Y443 | Phosphorylation | Uniprot | |
| T450 | Phosphorylation | Uniprot | |
| R452 | Methylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| K460 | Ubiquitination | Uniprot |
PTMs - Q15208/Q9Y2H1 酵素として
| Substrate | Site | Source |
|---|---|---|
| P38936 (CDKN1A) | S146 | Uniprot |
| P46937 (YAP1) | S61 | Uniprot |
| P46937 (YAP1) | S109 | Uniprot |
| P46937 (YAP1) | S127 | Uniprot |
| P46937 (YAP1) | S164 | Uniprot |
| Q02241 (KIF23) | S716 | Uniprot |
| Q02241 (KIF23) | S814 | Uniprot |
| Q15208 (STK38) | T74 | Uniprot |
| Q15208 (STK38) | S281 | Uniprot |
| Q15208 (STK38) | T444 | Uniprot |
| Q2M2I8 (AAK1) | S637 | Uniprot |
| Q96QF0 (RAB3IP) | S288 | Uniprot |
| Q96RD6 (PANX2) | S514 | Uniprot |
| Q9BXF6 (RAB11FIP5) | S307 | Uniprot |
| Q9UBF8 (PI4KB) | S277 | Uniprot |
研究背景
Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.
ISGylated.
Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.
Nucleus. Cytoplasm.
Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Homodimeric S100B binds two molecules of STK38. Interacts with MOB1 and MOB2. Interacts with MAP3K1 and MAP3K2 (via the kinase catalytic domain). Forms a tripartite complex with MOBKL1B and STK3/MST2. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4 (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Involved in the regulation of structural processes in differentiating and mature neuronal cells.
Cytoplasm. Cytoplasm>Cytoskeleton. Membrane.
Note: Associated with the actin cytoskeleton. Co-localizes with STK24/MST3 in the membrane.
Ubiquitously expressed with highest levels observed in the thymus.
Homodimeric S100B binds two molecules of STK38L. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4 (By similarity). Interacts with MOB1 and MOB2.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
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