Phospho-NuaK1 (Ser600) Antibody - #AF8175
| 製品: | Phospho-NuaK1 (Ser600) Antibody |
| カタログ: | AF8175 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-NuaK1 (Ser600) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 75KD; 74kD(Calculated). |
| ユニプロット: | O60285 |
| RRID: | AB_2840237 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8175, RRID:AB_2840237.
折りたたみ／展開
AMP activated protein kinase family member 5; AMPK related protein kinase 5; AMPK-related protein kinase 5; NUAK family SNF1 like kinase; NUAK family SNF1-like kinase 1; Nuak1; NUAK1_HUMAN; Omphalocele kinase 1; Probable serine/threonine protein kinase KIAA0537;
免疫原
Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines.
- O60285 NUAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGAAAPVAGDRPDLGLGAPGSPREAVAGATAALEPRKPHGVKRHHHKHNLKHRYELQETLGKGTYGKVKRATERFSGRVVAIKSIRKDKIKDEQDMVHIRREIEIMSSLNHPHIISIYEVFENKDKIVIIMEYASKGELYDYISERRRLSERETRHFFRQIVSAVHYCHKNGVVHRDLKLENILLDDNCNIKIADFGLSNLYQKDKFLQTFCGSPLYASPEIVNGRPYRGPEVDSWALGVLLYTLVYGTMPFDGFDHKNLIRQISSGEYREPTQPSDARGLIRWMLMVNPDRRATIEDIANHWWVNWGYKSSVCDCDALHDSESPLLARIIDWHHRSTGLQADTEAKMKGLAKPTTSEVMLERQRSLKKSKKENDFAQSGQDAVPESPSKLSSKRPKGILKKRSNSEHRSHSTGFIEGVVGPALPSTFKMEQDLCRTGVLLPSSPEAEVPGKLSPKQSATMPKKGILKKTQQRESGYYSSPERSESSELLDSNDVMGSSIPSPSPPDPARVTSHSLSCRRKGILKHSSKYSAGTMDPALVSPEMPTLESLSEPGVPAEGLSRSYSRPSSVISDDSVLSSDSFDLLDLQENRPARQRIRSCVSAENFLQIQDFEGLQNRPRPQYLKRYRNRLADSSFSLLTDMDDVTQVYKQALEICSKLN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60285 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | Q15831 (STK11) , Q9Y2H1 (STK38L) | Uniprot |
| R284 | Methylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S480 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S499 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S603 | Phosphorylation | Uniprot |
PTMs - O60285 酵素として
| Substrate | Site | Source |
|---|---|---|
| O14974 (PPP1R12A) | S445 | Uniprot |
| O14974 (PPP1R12A) | S472 | Uniprot |
| O14974 (PPP1R12A) | S910 | Uniprot |
| O95835 (LATS1) | S464 | Uniprot |
| P04637 (TP53) | S15 | Uniprot |
| P04637 (TP53) | S392 | Uniprot |
| P55212-1 (CASP6) | S257 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Not dephosphorylated by the myosin PP1 complex when regulating its activity, due to the presence of PPP1R12A, which prevents myosin PP1 from dephosphorylating NUAK1. Phosphorylated by STK38L upon stimulation with IGF1.
Nucleus. Cytoplasm.
Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines.
Interacts (via GILK motif) with PPP1CB; the interaction is direct and bridges NUAK1 and PPP1R12A. Interacts with CDKN1A.
The GILK motif mediates interaction with PPP1CB.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
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