Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody - #AF8176
| 製品: | Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody |
| カタログ: | AF8176 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-p38 gamma/delta (Tyr185/Tyr182) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 43KD; 42kD(Calculated). |
| ユニプロット: | P53778 | O15264 |
| RRID: | AB_2840238 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8176, RRID:AB_2840238.
折りたたみ／展開
ERK 6; ERK-6; ERK6; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK 12; Mapk12; Mitogen Activated Protein Kinase 12; Mitogen activated protein kinase p38 gamma; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase p38 gamma; MK12_HUMAN; P38 GAMMA; P38GAMMA; PRKM12; SAPK 3; SAPK-3; SAPK3; Stress Activated Protein Kinase 3; Stress-activated protein kinase 3; MAP kinase 13; MAP kinase p38 delta; MAPK 13; MAPK-13; Mapk13; MGC99536; Mitogen activated protein kinase 13; Mitogen-activated protein kinase 13; Mitogen-activated protein kinase p38 delta; MK13_HUMAN; OTTHUMP00000016282; OTTHUMP00000016283; p38 delta; P38delta; PRKM13; SAPK 4; SAPK4; Stress activated protein kinase 4; Stress-activated protein kinase 4;
免疫原
Highly expressed in skeletal muscle and heart.
O15264 MK13_HUMAN:Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
- P53778 MK12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSPPPARSGFYRQEVTKTAWEVRAVYRDLQPVGSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLSFKPPRQLGARVSKETPL
- O15264 MK13_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLIRKKGFYKQDVNKTAWELPKTYVSPTHVGSGAYGSVCSAIDKRSGEKVAIKKLSRPFQSEIFAKRAYRELLLLKHMQHENVIGLLDVFTPASSLRNFYDFYLVMPFMQTDLQKIMGMEFSEEKIQYLVYQMLKGLKYIHSAGVVHRDLKPGNLAVNEDCELKILDFGLARHADAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVGCIMAEMLTGKTLFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKDFTQLFPRASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQQPFDDSLEHEKLTVDEWKQHIYKEIVNFSPIARKDSRRRSGMKL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P53778/O15264 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | P53778 (MAPK12) | Uniprot |
| Y27 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | P46734 (MAP2K3) , P45985 (MAP2K4) , P52564 (MAP2K6) | Uniprot |
| Y185 | Phosphorylation | P46734 (MAP2K3) , P45985 (MAP2K4) , P52564 (MAP2K6) | Uniprot |
| K236 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y10 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | P52564 (MAP2K6) , P46734 (MAP2K3) | Uniprot |
| Y182 | Phosphorylation | P52564 (MAP2K6) , P46734 (MAP2K3) | Uniprot |
| T185 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot |
PTMs - P53778/O15264 酵素として
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S396 | Uniprot |
| O43464 (HTRA2) | S142 | Uniprot |
| O60239-2 (SH3BP5) | S194 | Uniprot |
| O60239-2 (SH3BP5) | S264 | Uniprot |
| O60239-1 (SH3BP5) | S351 | Uniprot |
| O60239-1 (SH3BP5) | S421 | Uniprot |
| P05787 (KRT8) | S74 | Uniprot |
| P10636-8 (MAPT) | T50 | Uniprot |
| P10636-8 (MAPT) | T69 | Uniprot |
| P10636-6 (MAPT) | T123 | Uniprot |
| P10636-2 (MAPT) | S144 | Uniprot |
| P10636-6 (MAPT) | T147 | Uniprot |
| P10636-8 (MAPT) | T153 | Uniprot |
| P10636-6 (MAPT) | T173 | Uniprot |
| P10636-8 (MAPT) | T181 | Uniprot |
| P10636-8 (MAPT) | S202 | Uniprot |
| P10636-8 (MAPT) | T205 | Uniprot |
| P10636-2 (MAPT) | S231 | Uniprot |
| P10636-8 (MAPT) | T231 | Uniprot |
| P10636-8 (MAPT) | S235 | Uniprot |
| P10636-6 (MAPT) | S262 | Uniprot |
| P10636-8 (MAPT) | S320 | Uniprot |
| P10636-8 (MAPT) | S396 | Uniprot |
| P10636-8 (MAPT) | S404 | Uniprot |
| P10636-8 (MAPT) | S422 | Uniprot |
| P11388 (TOP2A) | S1525 | Uniprot |
| P15172 (MYOD1) | S200 | Uniprot |
| P15172 (MYOD1) | S201 | Uniprot |
| P15336 (ATF2) | T69 | Uniprot |
| P15336 (ATF2) | T71 | Uniprot |
| P15336 (ATF2) | S90 | Uniprot |
| P16949 (STMN1) | S25 | Uniprot |
| P26045 (PTPN3) | S459 | Uniprot |
| P30281 (CCND3) | T283 | Uniprot |
| P30307 (CDC25C) | S207 | Uniprot |
| P35222 (CTNNB1) | S605 | Uniprot |
| P53778-1 (MAPK12) | S3 | Uniprot |
| P78352 (DLG4) | T287 | Uniprot |
| P78352 (DLG4) | S290 | Uniprot |
| Q12959 (DLG1) | S122 | Uniprot |
| Q12959 (DLG1) | S158 | Uniprot |
| Q12959 (DLG1) | T209 | Uniprot |
| Q12959 (DLG1) | S443 | Uniprot |
| Q12959 (DLG1) | Y447 | Uniprot |
| Q13424-1 (SNTA1) | S193 | Uniprot |
| Q13424 (SNTA1) | S201 | Uniprot |
| Q14934 (NFATC4) | S168 | Uniprot |
| Q14934 (NFATC4) | S170 | Uniprot |
| Q8IW41 (MAPKAPK5) | T182 | Uniprot |
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S359 | Uniprot |
| O00418 (EEF2K) | S396 | Uniprot |
| P04637 (TP53) | S33 | Uniprot |
| P04637 (TP53) | S46 | Uniprot |
| P05787 (KRT8) | S74 | Uniprot |
| P10636-8 (MAPT) | T50 | Uniprot |
| P10636-8 (MAPT) | T69 | Uniprot |
| P10636-8 (MAPT) | T153 | Uniprot |
| P10636-8 (MAPT) | S202 | Uniprot |
| P10636-8 (MAPT) | T205 | Uniprot |
| P10636-8 (MAPT) | T212 | Uniprot |
| P10636-8 (MAPT) | T217 | Uniprot |
| P10636-8 (MAPT) | S235 | Uniprot |
| P10636-8 (MAPT) | S396 | Uniprot |
| P10636-8 (MAPT) | S404 | Uniprot |
| P10636-8 (MAPT) | S422 | Uniprot |
| P16949 (STMN1) | S25 | Uniprot |
| P16949 (STMN1) | S38 | Uniprot |
| P30281 (CCND3) | T283 | Uniprot |
| P78352 (DLG4) | T287 | Uniprot |
| Q13501 (SQSTM1) | T269 | Uniprot |
| Q13501 (SQSTM1) | S272 | Uniprot |
| Q14934 (NFATC4) | S168 | Uniprot |
| Q14934 (NFATC4) | S170 | Uniprot |
| Q15139 (PRKD1) | S208 | Uniprot |
| Q15139 (PRKD1) | S249 | Uniprot |
| Q15139 (PRKD1) | S397 | Uniprot |
| Q15139 (PRKD1) | S401 | Uniprot |
| Q15139 (PRKD1) | S742 | Uniprot |
| Q8IW41 (MAPKAPK5) | T182 | Uniprot |
| Q8NHW3 (MAFA) | T57 | Uniprot |
| Q8NHW3 (MAFA) | T134 | Uniprot |
| Q8NHW3 (MAFA) | S336 | Uniprot |
研究背景
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme.
Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
Cytoplasm. Nucleus. Mitochondrion.
Note: Mitochondrial when associated with SH3BP5. In skeletal muscle colocalizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma (By similarity).
Highly expressed in skeletal muscle and heart.
Monomer. Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with SH3BP5. Interacts with LIN7C, SCRIB and SYNJ2BP (By similarity). Interacts with PTPN4; this interaction induces the activation of PTPN4 phosphatase activity.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.
Dually phosphorylated on Thr-180 and Tyr-182 by MAP2K3/MKK3, MAP2K4/MKK4, MAP2K6/MKK6 and MAP2K7/MKK7, which activates the enzyme. Dephosphorylated by dual specificity phosphatase DUSP1.
Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
Interacts with MAPK8IP2.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究領域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
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