Phospho-ZAK (Ser165) Antibody - #AF8195

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製品: Phospho-ZAK (Ser165) Antibody
カタログ: AF8195
タンパク質の説明: Rabbit polyclonal antibody to Phospho-ZAK (Ser165)
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 91 KD; 91kD(Calculated).
ユニプロット: Q9NYL2
RRID: AB_2840257

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
クローナリティ:
Polyclonal
特異性:
Phospho-ZAK (Ser165) Antibody detects endogenous levels of ZAK only when phosphorylated at Ser165.
RRID:
AB_2840257
引用形式: Affinity Biosciences Cat# AF8195, RRID:AB_2840257.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AZK; Cervical cancer suppressor gene 4 protein; HCCS 4; HCCS-4; HCCS4; Human cervical cancer suppressor gene 4 protein; Leucine zipper and sterile alpha motif containing kinase; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen activated protein kinase kinase kinase MLT; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase 7; Mixed lineage kinase related kinase; Mixed lineage kinase related kinase MRK beta; Mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; MLK 7; MLK like mitogen activated protein triple kinase; MLK related kinase; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MLK7; Mlklak; MLT; MLTK_HUMAN; MRK; Sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; Zak;

免疫原

免疫原:
Uniprot:

Q9NYL2(M3K20_HUMAN) >>Visit HPA database.

遺伝子(ID):
MAP3K20(51776)
発現特異性:
Q9NYL2 M3K20_HUMAN:

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.

タンパク質配列:
MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWRNF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYL2 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S3 Phosphorylation
S7 Phosphorylation
K34 Ubiquitination
K52 Ubiquitination
Y78 Phosphorylation
Y84 Phosphorylation
Y93 Phosphorylation
K116 Acetylation
Y120 Phosphorylation
K128 Ubiquitination
S136 Phosphorylation
S155 Phosphorylation
T161 Phosphorylation Q9NYL2 (MAP3K20)
T162 Phosphorylation Q9NYL2 (MAP3K20)
S165 Phosphorylation Q9NYL2 (MAP3K20)
K248 Ubiquitination
T267 Phosphorylation
S268 Phosphorylation
K272 Ubiquitination
S275 Phosphorylation
S302 Phosphorylation
K304 Ubiquitination
K309 Ubiquitination
K316 Ubiquitination
K354 Acetylation
S362 Phosphorylation
S366 Phosphorylation
K369 Acetylation
K376 Acetylation
K401 Ubiquitination
K538 Acetylation
K544 Acetylation
S557 Phosphorylation
S563 Phosphorylation
S565 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
T576 Phosphorylation
S584 Phosphorylation
T586 Phosphorylation
S591 Phosphorylation
S593 Phosphorylation
S599 Phosphorylation
S603 Phosphorylation
S610 Phosphorylation
T628 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
S636 Phosphorylation
S637 Phosphorylation
T639 Phosphorylation
S648 Phosphorylation
S649 Phosphorylation
S654 Phosphorylation
S660 Phosphorylation
S661 Phosphorylation
T666 Phosphorylation
S667 Phosphorylation
S668 Phosphorylation
R670 Methylation
Y673 Phosphorylation
S674 Phosphorylation
R683 Methylation
S685 Phosphorylation
S687 Phosphorylation
S690 Phosphorylation
S691 Phosphorylation
R693 Methylation
T704 Phosphorylation
R707 Methylation
R709 Methylation
K713 Methylation
S718 Phosphorylation
S720 Phosphorylation
S727 Phosphorylation
S733 Phosphorylation
S757 Phosphorylation
S761 Phosphorylation
S764 Phosphorylation
T769 Phosphorylation
K770 Ubiquitination
S781 Phosphorylation

PTMs - Q9NYL2 酵素として

Substrate Site Source
O96017 (CHEK2) T68 Uniprot
Q9NYL2 (MAP3K20) T161 Uniprot
Q9NYL2 (MAP3K20) T162 Uniprot
Q9NYL2 (MAP3K20) S165 Uniprot

研究背景

機能:

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Involved in limb development.

Phosphorylates histone H3 at 'Ser-28'. May have role in neoplastic cell transformation and cancer development. Causes cell shrinkage and disruption of actin stress fibers.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus upon ultraviolet B irradiation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.

サブユニット構造:

Homodimer. Interacts with PKN1 and ZNF33A. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, MAP3K20 and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and MAP3K20.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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