Phospho-eIF2B epsilon (Ser540) Antibody - #AF8203
| 製品: | Phospho-eIF2B epsilon (Ser540) Antibody |
| カタログ: | AF8203 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-eIF2B epsilon (Ser540) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog, Chicken |
| 分子量: | 80KD; 80kD(Calculated). |
| ユニプロット: | Q13144 |
| RRID: | AB_2840265 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8203, RRID:AB_2840265.
折りたたみ／展開
CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon;
免疫原
- Q13144 EI2BE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13144 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| K61 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S469 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| K481 | Ubiquitination | Uniprot | |
| Y483 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S540 | Phosphorylation | P49841 (GSK3B) , P49840 (GSK3A) | Uniprot |
| S544 | Phosphorylation | P49840 (GSK3A) , P49841 (GSK3B) | Uniprot |
| K565 | Ubiquitination | Uniprot | |
| Y583 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| C618 | S-Nitrosylation | Uniprot | |
| K693 | Ubiquitination | Uniprot | |
| S717 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| S718 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
研究背景
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.
Polyubiquitinated, probably by NEDD4.
Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.
Belongs to the eIF-2B gamma/epsilon subunits family.
研究領域
· Genetic Information Processing > Translation > RNA transport.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.