Phospho-AMPK beta 1 (Ser108) Antibody - #AF8238

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製品: Phospho-AMPK beta 1 (Ser108) Antibody
カタログ: AF8238
タンパク質の説明: Rabbit polyclonal antibody to Phospho-AMPK beta 1 (Ser108)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 38kDa; 30kD(Calculated).
ユニプロット: Q9Y478
RRID: AB_2840300

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%)
クローナリティ:
Polyclonal
特異性:
Phospho-AMPK beta 1 (Ser108) Antibody detects endogenous levels of AMPK beta 1 only when phosphorylated at Ser108.
RRID:
AB_2840300
引用形式: Affinity Biosciences Cat# AF8238, RRID:AB_2840300.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

1300015D22Rik; 5''-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase beta-1 subunit; AAKB1_HUMAN; AMP-activated protein kinase beta subunit; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1; AMPK; AMPK beta 1 chain; AMPK subunit beta-1; AMPK-BETA-1; AMPKb; AU021155; E430008F22; HAMPKb; MGC17785; PRKAB1; Protein kinase AMP activated non catalytic subunit beta 1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1;

免疫原

免疫原:
Uniprot:

Q9Y478(AAKB1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PRKAB1(5564)
タンパク質配列:
MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
90
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y478 基板として

Site PTM Type Enzyme
G2 Myristoylation
T4 Phosphorylation
S5 Phosphorylation
S6 Phosphorylation
T19 Phosphorylation
S24 Phosphorylation Q13131 (PRKAA1)
S25 Phosphorylation
T28 Phosphorylation
K35 Ubiquitination
S40 Phosphorylation
S49 Phosphorylation
K53 Ubiquitination
K57 Ubiquitination
K72 Ubiquitination
T80 Phosphorylation Q13131 (PRKAA1)
K89 Ubiquitination
Y92 Phosphorylation
S94 Phosphorylation
S96 Phosphorylation
S101 Phosphorylation
K102 Ubiquitination
S108 Phosphorylation Q13131 (PRKAA1)
S138 Phosphorylation
S144 Phosphorylation
T148 Phosphorylation Q13131 (PRKAA1)
K157 Ubiquitination
T158 Phosphorylation Q13131 (PRKAA1)
S170 Phosphorylation
S174 Phosphorylation Q13131 (PRKAA1)
S177 Phosphorylation Q13131 (PRKAA1)
S180 Phosphorylation
S181 Phosphorylation
S182 Phosphorylation
Y187 Phosphorylation
K195 Ubiquitination
S252 Phosphorylation
T254 Phosphorylation
Y261 Phosphorylation
K268 Ubiquitination

PTMs - Q9Y478 酵素として

Substrate Site Source
P14859 (POU2F1) S335 Uniprot
P29474 (NOS3) S1177 Uniprot

研究背景

機能:

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

PTMs:

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

サブユニット構造:

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

タンパク質ファミリー:

The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.

Belongs to the 5'-AMP-activated protein kinase beta subunit family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

参考文献

1). WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1. Cell Death and Disease, 2021 (PubMed: 33658485) [IF=9.0]

Application: WB    Species: mouse    Sample: WBP2 WT cells

Fig. 6 |The impact of WBP2 on AMPK is mediated by the phosphorylation of AMPKβ1 at Ser108.C Western blot assays were performed to detect the expression of AMPKα, p-AMPKα, ACC, and p-ACC in the WBP2 WT cells with or without WBP2 overexpression, with or without AMPKβ1 siRNA, and with or without AMPKβ1 WT or with the Ser108 to Ala108 mutation (S108A); anti-WBP2 was used to detect the expression of WBP2, and β-actin served as the loading control. Immunoprecipitation assays using anti-Flag and western blot assays using anti-phospho-(Ser/Thr) (Phos S/T) to detect the phosphorylation of AMPKβ1.

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