Phospho-BAG3 (Tyr457) Antibody - #AF8243
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8243, RRID:AB_2840305.
折りたたみ／展開
BAG 3; BAG family molecular chaperone regulator 3; BAG-3; Bag3; BAG3_HUMAN; Bcl 2 binding protein; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2 associated athanogene 3; BCL2 binding athanogene 3; BIS; CAIR 1; Docking protein CAIR 1; Docking protein CAIR-1; MFM6;
免疫原
- O95817 BAG3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAATHSPMMQVASGNGDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQCGQVAAAAAAQPPASHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95817 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| R90 | Methylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| R106 | Methylation | Uniprot | |
| Y114 | Phosphorylation | Uniprot | |
| R121 | Methylation | Uniprot | |
| R123 | Methylation | Uniprot | |
| R133 | Methylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| R139 | Methylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| C151 | S-Nitrosylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| C179 | S-Nitrosylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S190 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| R203 | Methylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| R218 | Methylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K230 | Methylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| R258 | Methylation | Uniprot | |
| R261 | Methylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| R267 | Methylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S289 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S291 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| K445 | Acetylation | Uniprot | |
| K445 | Sumoylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| Y451 | Phosphorylation | Uniprot | |
| Y457 | Phosphorylation | Uniprot | |
| T459 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| Y508 | Phosphorylation | Uniprot |
研究背景
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.
Nucleus. Cytoplasm.
Note: Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920).
Binds to the ATPase domain of HSP70/HSC70 chaperones. Interacts with BCL2. Interacts with phospholipase C-gamma proteins. Interacts with DNAJB6. Interacts (via BAG domain) with HSF1; this interaction occurs in normal and heat-shocked cells promoting HSF1 nucleocytoplasmic shuttling. Interacts with HSPA8 (via NBD). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD). Interacts (via WW domain 1) with SYNPO2 (via PPPY motif). Interacts with HSPB8.
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