Phospho-Caldesmon (Ser759) Antibody - #AF8250
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8250, RRID:AB_2840312.
折りたたみ／展開
CAD; CALD 1; CALD1; CALD1_HUMAN; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22;
免疫原
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
- Q05682 CALD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDFERRRELRRQKREEMRLEAERIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNAQNSVPDEEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQNDTAENETTEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQVEVMVEEKTTESQEETVVMSLKNGQISSEEPKQEEEREQGSDEISHHEKMEEEDKERAEAERARLEAEERERIKAEQDKKIADERARIEAEEKAAAQERERREAEERERMREEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRARAEEEEKAKVEEQKRNKQLEEKKHAMQETKIKGEKVEQKIEGKWVNEKKAQEDKLQTAVLKKQGEEKGTKVQAKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTEVKSQNGEFMTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDDKKPFKCFTPKGSSLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTSPTKV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q05682 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y27 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| T83 | Phosphorylation | Uniprot | |
| T84 | Phosphorylation | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K197 | Sumoylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| K427 | Acetylation | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| K534 | Acetylation | Uniprot | |
| T546 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| T638 | Phosphorylation | Uniprot | |
| K640 | Acetylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | P17252 (PRKCA) , Q9UQM7 (CAMK2A) | Uniprot |
| K645 | Acetylation | Uniprot | |
| K645 | Sumoylation | Uniprot | |
| K655 | Acetylation | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| S656 | Phosphorylation | P17252 (PRKCA) , Q9UQM7 (CAMK2A) | Uniprot |
| S660 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| T666 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| S673 | Phosphorylation | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| S677 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y682 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| K713 | Ubiquitination | Uniprot | |
| S714 | Phosphorylation | O75914 (PAK3) , Q13177 (PAK2) | Uniprot |
| K718 | Ubiquitination | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| T726 | Phosphorylation | Uniprot | |
| T730 | Phosphorylation | P24941 (CDK2) , Q00534 (CDK6) | Uniprot |
| K733 | Acetylation | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| T735 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| S744 | Phosphorylation | Q15746 (MYLK) , Q13177 (PAK2) , O75914 (PAK3) | Uniprot |
| K752 | Acetylation | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| K758 | Acetylation | Uniprot | |
| S759 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S765 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| K775 | Methylation | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| T788 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | P27361 (MAPK3) , Q00534 (CDK6) , P28482 (MAPK1) | Uniprot |
| T791 | Phosphorylation | Uniprot |
研究背景
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity).
Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril. Cytoplasm>Cytoskeleton>Stress fiber.
Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
Belongs to the caldesmon family.
研究領域
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
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