Phospho-Calpain 2 (Ser50) Antibody - #AF8251
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8251, RRID:AB_2840313.
折りたたみ／展開
Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; Calcium-activated neutral protease 2, catalytic subunit; Calcium-activated neutral proteinase 2; CALP80; Calpain 2 (m/II) large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large subunit; Calpain 2, large [catalytic] subunit; Calpain large polypeptide L2; Calpain M type; Calpain M-type; Calpain-2 catalytic subunit; Calpain-2 large subunit; Calpain2; CAN2_HUMAN; CANP 2; CANP L2; CANP2; CANPL 2; CANPL2; CANPml; Capa2; CAPN 2; CAPN2; FLJ39928; M calpain; M calpin; M type; M-calpain; mCANP; Millimolar calpain; Millimolar-calpain;
免疫原
- P17655 CAN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGIAAKLAKDREAAEGLGSHDRAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P17655 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K7 | Acetylation | Uniprot | |
| R23 | Methylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| C82 | S-Nitrosylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| K354 | Acetylation | Uniprot | |
| K355 | Acetylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| S369 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T370 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K390 | Sumoylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| C577 | S-Nitrosylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| Y625 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| K674 | Ubiquitination | Uniprot |
研究背景
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Cytoplasm. Cell membrane.
Note: Translocates to the plasma membrane upon Ca(2+) binding.
Ubiquitous.
Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Interacts with CPEB3; this leads to cleavage of CPEB3.
Belongs to the peptidase C2 family.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
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