Phospho-CEP55 (Ser425) Antibody - #AF8254
製品: | Phospho-CEP55 (Ser425) Antibody |
カタログ: | AF8254 |
タンパク質の説明: | Rabbit polyclonal antibody to Phospho-CEP55 (Ser425) |
アプリケーション: | WB |
反応性: | Human, Mouse |
分子量: | 54 KD; 54kD(Calculated). |
ユニプロット: | Q53EZ4 |
RRID: | AB_2840316 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8254, RRID:AB_2840316.
折りたたみ/展開
C10orf3; cancer/testis antigen 111; Centrosomal protein 55kDa; Centrosomal protein of 55 kDa; CEP 55; Cep55; CEP55_HUMAN; CT111; FLJ10540; Up regulated in colon cancer 6; Up-regulated in colon cancer 6; URCC 6; URCC6;
免疫原
Expressed in embryonic brain (PubMed:28264986). Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex (PubMed:28264986). Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level) (PubMed:28295209, PubMed:28264986). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
- Q53EZ4 CEP55_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTTLLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQMQACTLDFENEKLDRQHVQHQLHVILKELRKARNQITQLESLKQLHEFAITEPLVTFQGETENREKVAASPKSPTAALNESLVECPKCNIQYPATEHRDLLVHVEYCSK
PTMs - Q53EZ4 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S5 | Phosphorylation | Uniprot | |
K13 | Ubiquitination | Uniprot | |
K17 | Ubiquitination | Uniprot | |
S21 | Phosphorylation | Uniprot | |
K22 | Ubiquitination | Uniprot | |
S23 | Phosphorylation | Uniprot | |
K29 | Ubiquitination | Uniprot | |
K31 | Ubiquitination | Uniprot | |
K38 | Ubiquitination | Uniprot | |
S40 | Phosphorylation | Uniprot | |
K48 | Ubiquitination | Uniprot | |
T52 | Phosphorylation | Uniprot | |
K92 | Ubiquitination | Uniprot | |
K119 | Ubiquitination | Uniprot | |
K125 | Ubiquitination | Uniprot | |
K129 | Ubiquitination | Uniprot | |
S144 | Phosphorylation | Uniprot | |
K145 | Ubiquitination | Uniprot | |
T146 | Phosphorylation | Uniprot | |
T148 | Phosphorylation | Uniprot | |
S152 | Phosphorylation | Uniprot | |
T154 | Phosphorylation | Uniprot | |
K180 | Ubiquitination | Uniprot | |
K196 | Ubiquitination | Uniprot | |
K201 | Ubiquitination | Uniprot | |
K207 | Ubiquitination | Uniprot | |
K208 | Ubiquitination | Uniprot | |
S215 | Phosphorylation | Uniprot | |
K222 | Ubiquitination | Uniprot | |
Y228 | Phosphorylation | Uniprot | |
K233 | Ubiquitination | Uniprot | |
K235 | Ubiquitination | Uniprot | |
K245 | Ubiquitination | Uniprot | |
K246 | Ubiquitination | Uniprot | |
K268 | Ubiquitination | Uniprot | |
K274 | Ubiquitination | Uniprot | |
Y284 | Phosphorylation | Uniprot | |
S285 | Phosphorylation | Uniprot | |
K300 | Ubiquitination | Uniprot | |
K306 | Ubiquitination | Uniprot | |
K317 | Ubiquitination | Uniprot | |
Y336 | Phosphorylation | Uniprot | |
T337 | Phosphorylation | Uniprot | |
K341 | Ubiquitination | Uniprot | |
T360 | Phosphorylation | Uniprot | |
K367 | Ubiquitination | Uniprot | |
S396 | Phosphorylation | Uniprot | |
K398 | Ubiquitination | Uniprot | |
S425 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) | Uniprot |
K427 | Ubiquitination | Uniprot | |
S428 | Phosphorylation | P06493 (CDK1) , P28482 (MAPK1) | Uniprot |
T430 | Phosphorylation | Uniprot | |
S436 | Phosphorylation | P53350 (PLK1) | Uniprot |
K442 | Ubiquitination | Uniprot | |
Y461 | Phosphorylation | Uniprot | |
S463 | Phosphorylation | Uniprot |
研究背景
Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney.
There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cleavage furrow. Midbody>Midbody ring.
Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
Expressed in embryonic brain. Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex. Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry. Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding. Interacts with MVB12A, VPS37B, VPS37C and VPS28.
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