Phospho-Claspin (Ser30) Antibody - #AF8255
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8255, RRID:AB_2840317.
折りたたみ／展開
Claspin; claspin homolog (Xenopus laevis); Claspin homolog; CLSPN; CLSPN protein; CLSPN_HUMAN; hClaspin; Hu Claspin;
免疫原
- Q9HAW4 CLSPN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETEDTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRPSPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELKADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKESSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKFTSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDDSTSGLTRSIFKYLES
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9HAW4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S30 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | O00311 (CDC7) | Uniprot |
| T69 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | O00311 (CDC7) | Uniprot |
| Y120 | Phosphorylation | Uniprot | |
| K187 | Acetylation | Uniprot | |
| K188 | Acetylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S265 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S282 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K403 | Sumoylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K606 | Acetylation | Uniprot | |
| T639 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | O00311 (CDC7) | Uniprot |
| K708 | Ubiquitination | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S721 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S723 | Phosphorylation | Uniprot | |
| T724 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| S744 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S750 | Phosphorylation | Uniprot | |
| K756 | Acetylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S823 | Phosphorylation | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| K828 | Ubiquitination | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| Y843 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| Y887 | Phosphorylation | Uniprot | |
| K891 | Acetylation | Uniprot | |
| K891 | Ubiquitination | Uniprot | |
| T916 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S921 | Phosphorylation | Uniprot | |
| K925 | Acetylation | Uniprot | |
| K925 | Ubiquitination | Uniprot | |
| S945 | Phosphorylation | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| T949 | Phosphorylation | Uniprot | |
| S950 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| T955 | Phosphorylation | Uniprot | |
| S958 | Phosphorylation | Uniprot | |
| S959 | Phosphorylation | Uniprot | |
| K963 | Ubiquitination | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| S984 | Phosphorylation | Q9BTU6 (PI4K2A) | Uniprot |
| T987 | Phosphorylation | Uniprot | |
| S1012 | Phosphorylation | Uniprot | |
| S1018 | Phosphorylation | Uniprot | |
| S1020 | Phosphorylation | Uniprot | |
| S1129 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S1147 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S1156 | Phosphorylation | Uniprot | |
| T1161 | Phosphorylation | Uniprot | |
| S1204 | Phosphorylation | Uniprot | |
| S1223 | Phosphorylation | Uniprot | |
| K1265 | Ubiquitination | Uniprot | |
| T1287 | Phosphorylation | Uniprot | |
| S1289 | Phosphorylation | Uniprot | |
| K1292 | Sumoylation | Uniprot | |
| S1310 | Phosphorylation | Uniprot | |
| S1314 | Phosphorylation | Uniprot | |
| S1316 | Phosphorylation | Uniprot | |
| K1321 | Sumoylation | Uniprot | |
| K1321 | Ubiquitination | Uniprot | |
| T1322 | Phosphorylation | Uniprot |
研究背景
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
Nucleus.
Interacts (phosphorylation-dependent) with CHEK1; regulates CLSPN function in checkpoint for DNA damage and replication. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Interacts with TIMELESS.
The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
Belongs to the claspin family.
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