Phospho-Claudin 4 (Tyr208) Antibody - #AF8257

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製品: Phospho-Claudin 4 (Tyr208) Antibody
カタログ: AF8257
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Claudin 4 (Tyr208)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Monkey
分子量: 22 kDa; 22kD(Calculated).
ユニプロット: O14493
RRID: AB_2840319

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
クローナリティ:
Polyclonal
特異性:
Phospho-Claudin 4 (Tyr208) Antibody detects endogenous levels of Claudin 4 only when phosphorylated at Tyr208.
RRID:
AB_2840319
引用形式: Affinity Biosciences Cat# AF8257, RRID:AB_2840319.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Claudin-4; Claudin4; CLD4_HUMAN; CLDN 4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE R; CPE receptor; CPE-R; CPE-receptor; CPER; CPETR 1; CPETR; CPETR1; hCPE R; WBSCR8; Williams Beuren syndrome chromosome region 8 protein; Williams-Beuren syndrome chromosomal region 8 protein;

免疫原

免疫原:
Uniprot:

O14493(CLD4_HUMAN) >>Visit HPA database.

遺伝子(ID):
CLDN4(1364)
タンパク質配列:
MASMGLQVMGIALAVLGWLAVMLCCALPMWRVTAFIGSNIVTSQTIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALVIISIIVAALGVLLSVVGGKCTNCLEDESAKAKTMIVAGVVFLLAGLMVIVPVSWTAHNIIQDFYNPLVASGQKREMGASLYVGWAASGLLLLGGGLLCCNCPPRTDKPYSAKYSAARSAAASNYV

PTMs - O14493 基板として

Site PTM Type Enzyme
T189 Phosphorylation
Y193 Phosphorylation
S194 Phosphorylation P17252 (PRKCA)
Y197 Phosphorylation
S198 Phosphorylation
S202 Phosphorylation
S206 Phosphorylation
Y208 Phosphorylation P29317 (EPHA2)

研究背景

機能:

Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

PTMs:

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.

細胞の位置付け:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.
Note: CLDN4 is required for tight junction localization in the kidney.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Directly interacts with TJP1/ZO-1. Interacts with TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with EPHA2; phosphorylates CLDN4 and may regulate tight junctions. Interacts with CLDN1. Interacts with CLDN8 (By similarity).

(Microbial infection) Interacts (via both extracellular domains) with Clostridium perfringens enterotoxin CPE; the interaction may disrupt claudin assembly in tight junctions.

タンパク質ファミリー:

Belongs to the claudin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). 25-Hydroxycholesterol Exacerbated Experimental Colitis by Inhibiting Tissue Resident Memory ΓδT17 Cells Via Transcription Factor RORγt and Impairing the Mucosal Barrier.

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