Phospho-Calcipressin 1 (Ser163) Antibody - #AF8268

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製品: Phospho-Calcipressin 1 (Ser163) Antibody
カタログ: AF8268
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Calcipressin 1 (Ser163)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 22 KD; 28kD(Calculated).
ユニプロット: P53805
RRID: AB_2840330

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MS Report
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
Phospho-Calcipressin 1 (Ser163) Antibody detects endogenous levels of Calcipressin 1 only when phosphorylated at Ser163.
RRID:
AB_2840330
引用形式: Affinity Biosciences Cat# AF8268, RRID:AB_2840330.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Adapt 78; Adapt78; Calcipressin 1; Calcipressin-1; Calcium and oxidant inducible mRNA; CSP 1; CSP1; Down syndrome candidate region 1; Down syndrome critical region gene 1; Down syndrome critical region protein 1; DSC 1; DSC1; DSCR 1; DSCR1; MCIP 1; MCIP1; Modulatory calcineurin interacting protein 1; Myocyte enriched calcineurin interacting protein 1; Myocyte-enriched calcineurin-interacting protein 1; Near DSCR proline rich protein; RCAN1; RCAN1_HUMAN; RCN 1; RCN1; Regulator of calcineurin 1;

免疫原

免疫原:
Uniprot:

P53805(RCAN1_HUMAN) >>Visit HPA database.

遺伝子(ID):
RCAN1(1827)
発現特異性:
P53805 RCAN1_HUMAN:

Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.

タンパク質配列:
MEDGVAGPQLGAAAEAAEAAEARARPGVTLRPFAPLSGAAEADEGGGDWSFIDCEMEEVDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPEYTPIHLS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53805 基板として

Site PTM Type Enzyme
K86 Ubiquitination
Y94 Phosphorylation
K128 Ubiquitination
T129 Phosphorylation
K134 Ubiquitination
S163 Phosphorylation Q99759 (MAP3K3) , P49841 (GSK3B)
S167 Phosphorylation Q99759 (MAP3K3) , Q13627 (DYRK1A) , P27361 (MAPK3) , P28482 (MAPK1)
T179 Phosphorylation
Y188 Phosphorylation
K192 Ubiquitination
T208 Phosphorylation
S218 Phosphorylation
T241 Phosphorylation
T247 Phosphorylation Q13627 (DYRK1A)

研究背景

機能:

Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development (By similarity).

PTMs:

Phosphorylation increases its ability to inhibit calcineurin and decreases protein half-life.

組織特異性:

Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.

サブユニット構造:

Interacts with RAF1. Interacts with PPP3CA and PPP3R1.

タンパク質ファミリー:

Belongs to the RCAN family.

研究領域

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

参考文献

1). Mangiferin Inhibits PDGF-BB-Induced Proliferation and Migration of Rat Vascular Smooth Muscle Cells and Alleviates Neointimal Formation in Mice through the AMPK/Drp1 Axis. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34900084)

Application: WB    Species: Mouse    Sample:

Figure 4 Mangiferin alleviated PDGF-BB-induced mitochondrial fission and increase in ROS levels. (a) RSMCs were stained with mitotracker probe after treatment with different concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. The average length of mitochondria was quantified using ImageJ software. (b) Intracellular ROS levels were detected using flow cytometry after RSMCs were treated with mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. (c–e) Western blot was used to measure the protein expression levels of AMPK, pAMPKThr172, Drp1, and pDrp1Ser616 after RSMCs were treated with various concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. All assays were conducted in triplicate and all data presented as mean ± S.D. ∗P < 0.05, compared with the control group. #P < 0.05, compared with the PDGF-BB group.

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