RHOBTB1/2 Antibody - #AF0450

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製品: RHOBTB1/2 Antibody
カタログ: AF0450
タンパク質の説明: Rabbit polyclonal antibody to RHOBTB1/2
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
分子量: 83kDa; 83kD,79kD(Calculated).
ユニプロット: Q9BYZ6 | O94844
RRID: AB_2834314

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
RHOBTB1/2 Antibody detects endogenous levels of total RHOBTB1/2.
RRID:
AB_2834314
引用形式: Affinity Biosciences Cat# AF0450, RRID:AB_2834314.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DBC 2; Deleted in breast cancer 2; Deleted in breast cancer 2 gene protein; p83; RHBT2_HUMAN; Rho related BTB domain containing 2; Rho related BTB domain containing protein 2; Rho-related BTB domain-containing protein 2; RHOBTB 2; Rhobtb2; 1700008H16Rik; 3110048G13Rik; AI173445; AI573858; AV350930; KIAA0740; MGC107538; MGC33059; MGC33841; OTTHUMP00000019662; OTTHUMP00000019663; RHBT1_HUMAN; Rho related BTB domain containing 1; Rho related BTB domain containing protein 1; Rho-related BTB domain-containing protein 1; Rhobtb1;

免疫原

免疫原:

A synthesized peptide derived from human RHBT2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9BYZ6(RHBT2_HUMAN) >>Visit HPA database.

O94844(RHBT1_HUMAN) >>Visit HPA database.

遺伝子(ID):
RHOBTB1(9886) | RHOBTB2(23221)
発現特異性:
Q9BYZ6 RHBT2_HUMAN:

Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.

O94844 RHBT1_HUMAN:

Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.

タンパク質の説明:
RhoBTB2 Belongs to the small GTPase superfamily. Rho family.
タンパク質配列:
MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFAHKIYLSTSSSKFYDLFLMDLSEGELGGPSEPGGTHPEDHQGHSDQHHHHHHHHHGRDFLLRAASFDVCESVDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTYKSRLMVVVKMDSSIQPGPFRAVLKYLYTGELDENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYTGMFTSSPDLDDMKLIILANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADWCLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLKRQPKRRWLFWNSPSSPSSSAASSSSPSSSSAVV

MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSNKSLVEALGLEAEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYTGQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFKLDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELIALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA

研究背景

組織特異性:

Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.

タンパク質ファミリー:

Belongs to the small GTPase superfamily. Rho family.

組織特異性:

Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.

タンパク質ファミリー:

Belongs to the small GTPase superfamily. Rho family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

参考文献

1). LY86 facilitates ox-LDL-induced lipid accumulation in macrophages by upregulating SREBP2/HMGCR expression. BMC cardiovascular disorders, 2024 (PubMed: 38822281) [IF=2.0]

Application: WB    Species: human    Sample: THP-1 cells

Fig. 4 LY86 effectively attenuates ox-LDL-induced lipid accumulation in THP-1 macrophages. (A) Representative images of oil red O staining in THP-1 cells (×40 magnification). (B, C) The cropped blot and quantification of intracellular HMGCR, SREBP2, GRP78, and LY86 after 24-hour knockdown treatment with si-LY86 followed by 24-hour stimulation with ox-LDL; groups 1–4 represent the following conditions: 1 si-NC; 2 si-NC + ox-LDL; 3 si-LY86; and 4 si-LY86 + ox-LDL. Data are presented as mean ± SD (n = 3); *P

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