Phospho-Kizuna (Thr379) Antibody - #AF8277
| 製品: | Phospho-Kizuna (Thr379) Antibody |
| カタログ: | AF8277 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Kizuna (Thr379) |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Rat, Pig, Xenopus |
| 分子量: | 75KD; 75kD(Calculated). |
| ユニプロット: | Q2M2Z5 |
| RRID: | AB_2840339 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8277, RRID:AB_2840339.
折りたたみ／展開
Centrosomal protein kizuna; DKFZP586H021; HT013; Kiz; KIZ_HUMAN; KIZUNA; MGC102941; MGC141930; NCRNA00153; Plk1s1; Polo-like kinase 1 substrate 1; Uncharacterized protein C20orf19;
免疫原
- Q2M2Z5 KIZ_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRTLASAVPLSSPDYYERLGQLQHGLRDSEKKRLDLEKKLYEYNQSDTCRVKLKYVKLKNYLKEICESEKKAHTRNQEYLKRFERVQAHVVHFTTNTEKLQKLKLEYETQIKKMLCSKDSLGLKEELTDEDREKVAVHEGINSGTAMSRGLYQPATIFMGRQMSAILSMRDFSTEHKSPQPTKNFSIPDPHSHRQTAQSSNVTDSCVVQTSNDTQCLNKSDNIDGKASLQIGEKMPVTASVLSEEEQTHCLEIGSNTRHGKSNLSEGKKSAELNSPLRERLSPENRTTDLKCDSSSGSEGEILTREHIEVEEKRASPPVSPIPVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q2M2Z5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K40 | Ubiquitination | Uniprot | |
| Y42 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T379 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S435 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| T605 | Phosphorylation | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S619 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot |
研究背景
Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.
Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2.
Belongs to the kizuna family.
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