Phospho-MAP2 (Ser136) Antibody - #AF8281

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製品: Phospho-MAP2 (Ser136) Antibody
カタログ: AF8281
タンパク質の説明: Rabbit polyclonal antibody to Phospho-MAP2 (Ser136)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 55-75(2c/2d),280(2a/2b)kd; 200kD(Calculated).
ユニプロット: P11137
RRID: AB_2840343

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-MAP2 (Ser136) Antibody detects endogenous levels of MAP2 only when phosphorylated at Ser136.
RRID:
AB_2840343
引用形式: Affinity Biosciences Cat# AF8281, RRID:AB_2840343.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp686I2148; MAP 2; MAP dendrite specific; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; MTAP2_HUMAN;

免疫原

免疫原:
Uniprot:

P11137(MTAP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
MAP2(4133)
タンパク質配列:
MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTYPDKKDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKDEWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFLQPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEAINQETVQQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSEQKDQEPTTDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGAATSAELDMPFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQTGKESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLGGRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCFPVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSETVVEDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFYEGTDDKVRRDLATDLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEKSEEHADSKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVEQGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTKVSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEIPCPPAVSEADLATDERADVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQGEYDKLLFRSDTLQITDLGVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRFAALEQPEVERRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDDSIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKEPSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11137 基板として

Site PTM Type Enzyme
Y50 Phosphorylation
S63 Phosphorylation
T66 Phosphorylation
Y67 Phosphorylation P07948 (LYN) , P06241 (FYN)
S136 Phosphorylation
T168 Phosphorylation
T216 Phosphorylation
S233 Phosphorylation
T241 Phosphorylation
T258 Phosphorylation
S285 Phosphorylation
T290 Phosphorylation
S311 Phosphorylation
S315 Phosphorylation
S321 Phosphorylation
T323 Phosphorylation
S357 Phosphorylation
T361 Phosphorylation
S385 Phosphorylation Q9P0L2 (MARK1)
T427 Phosphorylation
T516 Phosphorylation
S532 Phosphorylation
S585 Phosphorylation
Y592 Phosphorylation
Y593 Phosphorylation
S610 Phosphorylation
S629 Phosphorylation
S725 Phosphorylation
S785 Phosphorylation
S788 Phosphorylation
S821 Phosphorylation
S823 Phosphorylation
S833 Phosphorylation
S843 Phosphorylation
T860 Phosphorylation
S862 Phosphorylation
S881 Phosphorylation
S913 Phosphorylation
Y996 Phosphorylation
S1005 Phosphorylation
S1021 Phosphorylation
S1031 Phosphorylation
S1106 Phosphorylation
T1108 Phosphorylation
S1139 Phosphorylation
T1154 Phosphorylation
S1155 Phosphorylation
S1158 Phosphorylation
S1234 Phosphorylation
S1324 Phosphorylation
S1347 Phosphorylation
S1353 Phosphorylation
T1366 Phosphorylation
T1368 Phosphorylation
Y1369 Phosphorylation
S1398 Phosphorylation
S1420 Phosphorylation
S1421 Phosphorylation
K1427 Acetylation
T1440 Phosphorylation
K1447 Ubiquitination
K1448 Ubiquitination
S1480 Phosphorylation
Y1493 Phosphorylation
T1494 Phosphorylation
T1497 Phosphorylation
S1500 Phosphorylation
K1505 Acetylation
T1506 Phosphorylation
K1524 Acetylation
S1534 Phosphorylation
S1539 Phosphorylation
S1540 Phosphorylation
S1546 Phosphorylation
S1555 Phosphorylation
S1562 Phosphorylation
S1564 Phosphorylation
S1588 Phosphorylation
T1590 Phosphorylation
S1591 Phosphorylation
T1592 Phosphorylation
T1594 Phosphorylation
T1595 Phosphorylation
S1598 Phosphorylation
T1599 Phosphorylation
T1602 Phosphorylation
T1605 Phosphorylation
S1608 Phosphorylation
Y1609 Phosphorylation
S1611 Phosphorylation
T1613 Phosphorylation
T1616 Phosphorylation
T1619 Phosphorylation
S1621 Phosphorylation
Y1622 Phosphorylation
T1628 Phosphorylation
T1631 Phosphorylation
S1634 Phosphorylation
S1640 Phosphorylation
T1649 Phosphorylation
S1653 Phosphorylation
T1656 Phosphorylation
S1675 Phosphorylation
S1679 Phosphorylation P17612 (PRKACA) , Q9P0L2 (MARK1)
T1680 Phosphorylation
S1702 Phosphorylation
S1706 Phosphorylation
S1710 Phosphorylation Q9P0L2 (MARK1) , P17612 (PRKACA)
S1727 Phosphorylation
S1742 Phosphorylation P17612 (PRKACA)
T1780 Phosphorylation
S1782 Phosphorylation P17612 (PRKACA)
S1786 Phosphorylation
S1787 Phosphorylation
S1790 Phosphorylation
S1795 Phosphorylation
S1798 Phosphorylation
S1799 Phosphorylation
S1800 Phosphorylation
S1802 Phosphorylation
S1808 Phosphorylation
T1813 Phosphorylation
T1819 Phosphorylation

研究背景

機能:

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

PTMs:

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites.

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