Phospho-NACA (Thr159) Antibody - #AF8291
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8291, RRID:AB_2840353.
折りたたみ／展開
Alpha NAC; Alpha-NAC; Hom s 2.02; HSD48; MGC117224; NAC-alpha; naca; NACA/1.9.2; NACA_HUMAN; NACA1; Nascent polypeptide associated complex alpha polypeptide; Nascent polypeptide associated complex alpha subunit; Nascent polypeptide associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha; Alpha NAC like; ANAC; Hom s 2.01; MGC71999; NAC alpha like; NACA2; NACAL; nascent polypeptide associated complex alpha polypeptide like; Nascent polypeptide associated complex subunit alpha 2;
免疫原
A synthesized peptide derived from human NACA around the phosphorylation site of Thr159.
Ubiquitously expressed.
Q9H009 NACA2_HUMAN:Expressed specifically in testis and skeletal muscle.
- Q13765 NACA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM
- Q9H009 NACA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGEATETVPATEQELPQSQAETGSGTASDSGESVPGIEEQDSTQTTTQKAWLVAAAEIDEEPVGKAKQSRSEKRARKAMSKLGLLQVTGVTRVTIWKSKNILFVITKLDVYKSPASDAYIVFGEAKIQDLSQQAQLAAAEKFRVQGEAVGNIQENTQTPTVQEESEEEEVDETGVEVKDVKLVMSQANVSRAKAVRALKNNSNDIVNAIMELTV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13765/Q9H009 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S43 | Phosphorylation | Q13418 (ILK) | Uniprot |
| S132 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K68 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| T214 | Phosphorylation | Uniprot |
研究背景
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
Cytoplasm. Nucleus.
Note: The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.
Ubiquitously expressed.
Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA.
The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA.
Belongs to the NAC-alpha family.
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity).
Cytoplasm. Nucleus.
Expressed specifically in testis and skeletal muscle.
Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains (By similarity).
Belongs to the NAC-alpha family.
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