Phospho-PDE4D (Ser715) Antibody - #AF8298
| 製品: | Phospho-PDE4D (Ser715) Antibody |
| カタログ: | AF8298 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-PDE4D (Ser715) |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 91kDa; 91kD(Calculated). |
| ユニプロット: | Q08499 |
| RRID: | AB_2840360 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8298, RRID:AB_2840360.
折りたたみ／展開
cAMP specific 3',5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; Pde4d; PDE4D_HUMAN; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D, cAMP specific (dunce); STRK1;
免疫原
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
- Q08499 PDE4D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQPQCPLQPPPPPPLPPPPPPPGAARGRYASSGATGRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q08499 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K309 | Methylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K387 | Sumoylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S746 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Uniprot |
研究背景
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.
Apical cell membrane. Cytoplasm. Membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells.
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
研究領域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Metabolism > Nucleotide metabolism > Purine metabolism.
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