Phospho-RAD23 (Ser357) Antibody - #AF8307
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8307, RRID:AB_2840369.
折りたたみ／展開
hHR 23A; hHR23A; HR23A; MGC111083; RAD 23a; RAD23 homolog A (S. cerevisiae); RAD23 homolog A; RAD23 yeast homolog A; RAD23A; RD23A_HUMAN; UV excision repair protein RAD23; UV excision repair protein RAD23 homolog A;
免疫原
- P54725 RD23A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54725 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot |
研究背景
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
(Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
Nucleus.
Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with UBQLN2.
(Microbial infection) Interacts with HIV-1 Vpr.
The ubiquitin-like domain mediates interaction with ATXN3.
The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
Belongs to the RAD23 family.
研究領域
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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