BHLHB3 Antibody - #AF0442
(1)
(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0442, RRID:AB_2834318.
折りたたみ／展開
Basic helix-loop-helix domain containing class B3; BHE41_HUMAN; bHLH protein DEC2; bHLHB3; bHLHe41; Class B basic helix loop helix protein 3; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; DEC2; Differentially expressed in chondrocytes protein 2; Enhancer of split and hairy related protein 1; Enhancer-of-split and hairy-related protein 1; hDEC2; Moderately similar to basic-helix loop helix protein M.musculus; SHARP-1;
免疫原
Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
- Q9C0J9 BHE41_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAKEVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKLDSRGGGSGGGPGGGAAAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLPFCFLSPSAAAAYVQPFLDKSGLEKYLYPAAAAAPFPLLYPGIPAPAAAAAAAAAAAAAAAAFPCLSSVLSPPPEKAGAAAATLLPHEVAPLGAPHPQHPHGRTHLPFAGPREPGNPESSAQEDPSQPGKEAP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9C0J9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K42 | Acetylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| K210 | Sumoylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K266 | Sumoylation | Uniprot | |
| S274 | Phosphorylation | Uniprot |
研究背景
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).
Nucleus.
Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
Homodimer (By similarity). Heterodimer with BHLHE40/DEC1. Interacts with CIART and ARNTL/BMAL1 (By similarity). Interacts with RXRA. Interacts with NR0B2 and HNF1A (By similarity).
研究領域
· Organismal Systems > Environmental adaptation > Circadian rhythm. (View pathway)
参考文献
Application: WB Species: Human Sample: colon cancer (CC) cells
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