Phospho-USF1 (Thr153) Antibody - #AF8331
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8331, RRID:AB_2840393.
折りたたみ／展開
bHLHb11; Class B basic helix-loop-helix protein 11; FCHL 1; FCHL; FCHL1; HGNC:12593; HYPLIP 1; HYPLIP1; Major late transcription factor 1; Major late transcription factor; MLTF; MLTF I; MLTFI; Transcription factor USF-1; UEF; Upstream stimulatory factor 1; Upstream transcription factor 1; Upstream transcription factor 1, isoform CRA_a; USF 1; USF;
免疫原
- P22415 USF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P22415 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T100 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | P49840 (GSK3A) , Q16539 (MAPK14) | Uniprot |
| S186 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S189 | Phosphorylation | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| K286 | Ubiquitination | Uniprot | |
| K288 | Sumoylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K306 | Sumoylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
研究背景
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Nucleus.
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.