Phospho-USP28 (Ser714) Antibody - #AF8333
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8333, RRID:AB_2840395.
折りたたみ／展開
Deubiquitinating enzyme 28; KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin carboxyl-terminal hydrolase 28; ubiquitin carboxyl-terminal hydrolase 28 variant 1; Ubiquitin specific peptidase 28; Ubiquitin specific processing protease 28; Ubiquitin specific protease 28; Ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; UBP28_HUMAN; USP 28; USP28; USP28 protein;
免疫原
- Q96RU2 UBP28_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK
PTMs - Q96RU2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K45 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T70 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| Y447 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S503 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| S550 | Phosphorylation | Uniprot | |
| Y654 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S720 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T753 | Phosphorylation | Uniprot | |
| T788 | Phosphorylation | Uniprot | |
| K839 | Ubiquitination | Uniprot | |
| K853 | Ubiquitination | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| Y901 | Phosphorylation | Uniprot | |
| T1048 | Phosphorylation | Uniprot | |
| S1053 | Phosphorylation | Uniprot | |
| S1059 | Phosphorylation | Uniprot |
研究背景
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
Degraded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1.
Nucleus>Nucleoplasm.
Interacts with ZNF304. Interacts with PRKD1. Interacts with TP53BP1. Interacts with isoform 1 of FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of MYC.
Belongs to the peptidase C19 family. USP28 subfamily.
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