Phospho-XPA (Ser196) Antibody - #AF8335
| 製品: | Phospho-XPA (Ser196) Antibody |
| カタログ: | AF8335 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-XPA (Ser196) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Monkey |
| 予測: | Pig, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 40kDa; 31kD(Calculated). |
| ユニプロット: | P23025 |
| RRID: | AB_2840397 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8335, RRID:AB_2840397.
折りたたみ／展開
DNA repair protein complementing XP A cells; DNA repair protein complementing XP-A cells; DNA repair protein complementing XPA cells; Excision repair controlling; Xeroderma pigmentosum 1; Xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A complementing protein; Xeroderma pigmentosum group A-complementing protein; XP 1; XP1; xpa; XPA_HUMAN; Xpac;
免疫原
- P23025 XPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P23025 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y48 | Phosphorylation | Uniprot | |
| K63 | Sumoylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K86 | Sumoylation | Uniprot | |
| K141 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K145 | Sumoylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | P78527 (PRKDC) , Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| S196 | Phosphorylation | P78527 (PRKDC) , Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| K204 | Ubiquitination | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| Y257 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot |
研究背景
Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage.
Ubiquitinated by HERC2 leading to degradation by the proteasome.
Nucleus.
Expressed in various cell lines and in skin fibroblasts.
Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2.
Belongs to the XPA family.
研究領域
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
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