XRN2 Antibody - #AF0490
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0490, RRID:AB_2834320.
折りたたみ／展開
5' 3' exoribonuclease 2; DHM1 like protein; DHP protein; XRN 2;
免疫原
Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.
- Q9H0D6 XRN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIHPCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFRASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIADRLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLGLATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGEFIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAIDRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKEKRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPSISPNTSFTSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQSYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMGVFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGIQGKFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAVLKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVTYQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQNAAFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0D6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K40 | Ubiquitination | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| R120 | Methylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| K132 | Sumoylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| T374 | Phosphorylation | Uniprot | |
| T379 | Phosphorylation | Uniprot | |
| T433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| T439 | Phosphorylation | P50750 (CDK9) , P50613 (CDK7) , P28482 (MAPK1) | Uniprot |
| S445 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| R458 | Methylation | Uniprot | |
| Y462 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| T481 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| K524 | Methylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| K640 | Acetylation | Uniprot | |
| K640 | Ubiquitination | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| Y666 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| Y764 | Phosphorylation | Uniprot | |
| K767 | Ubiquitination | Uniprot | |
| K776 | Ubiquitination | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| S790 | Phosphorylation | Uniprot | |
| K796 | Acetylation | Uniprot | |
| K796 | Ubiquitination | Uniprot | |
| R806 | Methylation | Uniprot | |
| R816 | Methylation | Uniprot | |
| R824 | Methylation | Uniprot | |
| R847 | Methylation | Uniprot | |
| R851 | Methylation | Uniprot | |
| K858 | Methylation | Uniprot | |
| S861 | Phosphorylation | Uniprot | |
| R864 | Methylation | Uniprot | |
| R870 | Methylation | Uniprot | |
| R880 | Methylation | Uniprot | |
| R883 | Methylation | Uniprot | |
| R895 | Methylation | Uniprot | |
| R921 | Methylation | Uniprot | |
| R922 | Methylation | Uniprot | |
| R925 | Methylation | Uniprot | |
| R928 | Methylation | Uniprot | |
| Y931 | Phosphorylation | Uniprot | |
| R933 | Methylation | Uniprot | |
| R946 | Methylation | Uniprot |
研究背景
Possesses 5'->3' exoribonuclease activity (By similarity). May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites.
Nucleus>Nucleolus.
Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.
Interacts with POLR2A and SMN1/SMN2. Interacts with CDKN2AIP and NKRF. Interacts with CDKN2AIPNL; the interaction is direct. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner.
Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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