Phospho-DOK2 (Tyr345) Antibody - #AF8345
| 製品: | Phospho-DOK2 (Tyr345) Antibody |
| カタログ: | AF8345 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-DOK2 (Tyr345) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Dog |
| 分子量: | 56kDa; 45kD(Calculated). |
| ユニプロット: | O60496 |
| RRID: | AB_2840407 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8345, RRID:AB_2840407.
折りたたみ／展開
Docking protein 2 56kDa; Docking protein 2; DOK 2; DOK R; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2); p56dok 2; p56DOK; p56dok2;
免疫原
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
- O60496 DOK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVIRLSDCLRVAEAGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKELSGPEGKQSRPCMEENELYSSAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALELWGGPEPGTQLYDWPYRFLRRFGRDKVTFSFEAGRRCVSGEGNFEFETRQGNEIFLALEEAISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPPPSPTTPVPAPRPRGQEGEYAVPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEGVAALSLYDSPQEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60496 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K7 | Ubiquitination | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| Y139 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P12931 (SRC) | Uniprot |
| S277 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | P08631 (HCK) , P07948 (LYN) , P12931 (SRC) | Uniprot |
| S309 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| Y330 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| Y345 | Phosphorylation | P07948 (LYN) , P08631 (HCK) , P12931 (SRC) | Uniprot |
| Y357 | Phosphorylation | Uniprot | |
| Y402 | Phosphorylation | Uniprot |
研究背景
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).
On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).
(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein UL46; this interaction induces DOK2 phosphorylation and subsequent degradation.
PTB domain mediates receptor interaction.
Belongs to the DOK family. Type A subfamily.
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