Collagen XII alpha 1 Antibody - #AF0741
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0741, RRID:AB_2834322.
折りたたみ／展開
BA209D8.1; COCA1; COL12A1; COL12A1L; Collagen alpha-1(XII) chain; collagen type XII proteoglycan; collagen, type XII, alpha 1; DJ234P15.1;
免疫原
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM.
- Q99715 COCA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRSRLPPALAALGAALLLSSIEAEVDPPSDLNFKIIDENTVHMSWAKPVDPIVGYRITVDPTTDGPTKEFTLSASTTETLLSELVPETEYVVTITSYDEVEESVPVIGQLTIQTGSSTKPVEKKPGKTEIQKCSVSAWTDLVFLVDGSWSVGRNNFKYILDFIAALVSAFDIGEEKTRVGVVQYSSDTRTEFNLNQYYQRDELLAAIKKIPYKGGNTMTGDAIDYLVKNTFTESAGARVGFPKVAIIITDGKSQDEVEIPARELRNVGVEVFSLGIKAADAKELKQIASTPSLNHVFNVANFDAIVDIQNEIISQVCSGVDEQLGELVSGEEVVEPPSNLIAMEVSSKYVKLNWNPSPSPVTGYKVILTPMTAGSRQHALSVGPQTTTLSVRDLSADTEYQISVSAMKGMTSSEPISIMEKTQPMKVQVECSRGVDIKADIVFLVDGSYSIGIANFVKVRAFLEVLVKSFEISPNRVQISLVQYSRDPHTEFTLKKFTKVEDIIEAINTFPYRGGSTNTGKAMTYVREKIFVPSKGSRSNVPKVMILITDGKSSDAFRDPAIKLRNSDVEIFAVGVKDAVRSELEAIASPPAETHVFTVEDFDAFQRISFELTQSICLRIEQELAAIKKKAYVPPKDLSFSEVTSYGFKTNWSPAGENVFSYHITYKEAAGDDEVTVVEPASSTSVVLSSLKPETLYLVNVTAEYEDGFSIPLAGEETTEEVKGAPRNLKVTDETTDSFKITWTQAPGRVLRYRIIYRPVAGGESREVTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAGDALFGEGTTLEERGSPQDLVTKDITDTSIGAYWTSAPGMVRGYRVSWKSLYDDVDTGEKNLPEDAIHTMIENLQPETKYRISVFATYSSGEGEPLTGDATTELSQDSKTLKVDEETENTMRVTWKPAPGKVVNYRVVYRPHGRGKQMVAKVPPTVTSTVLKRLQPQTTYDITVLPIYKMGEGKLRQGSGTTASRFKSPRNLKTSDPTMSSFRVTWEPAPGEVKGYKVTFHPTGDDRRLGELVVGPYDNTVVLEELRAGTTYKVNVFGMFDGGESSPLVGQEMTTLSDTTVMPILSSGMECLTRAEADIVLLVDGSWSIGRANFRTVRSFISRIVEVFDIGPKRVQIALAQYSGDPRTEWQLNAHRDKKSLLQAVANLPYKGGNTLTGMALNFIRQQNFRTQAGMRPRARKIGVLITDGKSQDDVEAPSKKLKDEGVELFAIGIKNADEVELKMIATDPDDTHAYNVADFESLSRIVDDLTINLCNSVKGPGDLEAPSNLVISERTHRSFRVSWTPPSDSVDRYKVEYYPVSGGKRQEFYVSRMETSTVLKDLKPETEYVVNVYSVVEDEYSEPLKGTEKTLPVPVVSLNIYDVGPTTMHVQWQPVGGATGYILSYKPVKDTEPTRPKEVRLGPTVNDMQLTDLVPNTEYAVTVQAVLHDLTSEPVTVREVTLPLPRPQDLKLRDVTHSTMNVFWEPVPGKVRKYIVRYKTPEEDVKEVEVDRSETSTSLKDLFSQTLYTVSVSAVHDEGESPPVTAQETTRPVPAPTNLKITEVTSEGFRGTWDHGASDVSLYRITWAPFGSSDKMETILNGDENTLVFENLNPNTIYEVSITAIYPDESESDDLIGSERTLPILTTQAPKSGPRNLQVYNATSNSLTVKWDPASGRVQKYRITYQPSTGEGNEQTTTIGGRQNSVVLQKLKPDTPYTITVSSLYPDGEGGRMTGRGKTKPLNTVRNLRVYDPSTSTLNVRWDHAEGNPRQYKLFYAPAAGGPEELVPIPGNTNYAILRNLQPDTSYTVTVVPVYTEGDGGRTSDTGRTLMRGLARNVQVYNPTPNSLDVRWDPAPGPVLQYRVVYSPVDGTRPSESIVVPGNTRMVHLERLIPDTLYSVNLVALYSDGEGNPSPAQGRTLPRSGPRNLRVFGETTNSLSVAWDHADGPVQQYRIIYSPTVGDPIDEYTTVPGRRNNVILQPLQPDTPYKITVIAVYEDGDGGHLTGNGRTVGLLPPQNIHISDEWYTRFRVSWDPSPSPVLGYKIVYKPVGSNEPMEAFVGEMTSYTLHNLNPSTTYDVNVYAQYDSGLSVPLTDQGTTLYLNVTDLKTYQIGWDTFCVKWSPHRAATSYRLKLSPADGTRGQEITVRGSETSHCFTGLSPDTDYGVTVFVQTPNLEGPGVSVKEHTTVKPTEAPTEPPTPPPPPTIPPARDVCKGAKADIVFLTDASWSIGDDNFNKVVKFIFNTVGGFDEISPAGIQVSFVQYSDEVKSEFKLNTYNDKALALGALQNIRYRGGNTRTGKALTFIKEKVLTWESGMRKNVPKVLVVVTDGRSQDEVKKAALVIQQSGFSVFVVGVADVDYNELANIASKPSERHVFIVDDFESFEKIEDNLITFVCETATSSCPLIYLDGYTSPGFKMLEAYNLTEKNFASVQGVSLESGSFPSYSAYRIQKNAFVNQPTADLHPNGLPPSYTIILLFRLLPETPSDPFAIWQITDRDYKPQVGVIADPSSKTLSFFNKDTRGEVQTVTFDTEEVKTLFYGSFHKVHIVVTSKSVKIYIDCYEIIEKDIKEAGNITTDGYEILGKLLKGERKSAAFQIQSFDIVCSPVWTSRDRCCDIPSRRDEGKCPAFPNSCTCTQDSVGPPGPPGPAGGPGAKGPRGERGISGAIGPPGPRGDIGPPGPQGPPGPQGPNGLSIPGEQGRQGMKGDAGEPGLPGRTGTPGLPGPPGPMGPPGDRGFTGKDGAMGPRGPPGPPGSPGSPGVTGPSGKPGKPGDHGRPGPSGLKGEKGDRGDIASQNMMRAVARQVCEQLISGQMNRFNQMLNQIPNDYQSSRNQPGPPGPPGPPGSAGARGEPGPGGRPGFPGTPGMQGPPGERGLPGEKGERGTGSSGPRGLPGPPGPQGESRTGPPGSTGSRGPPGPPGRPGNSGIRGPPGPPGYCDSSQCASIPYNGQGYPGSG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99715 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T67 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| K535 | Acetylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| S765 | Phosphorylation | Uniprot | |
| T778 | Phosphorylation | Uniprot | |
| T786 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| K1003 | Ubiquitination | Uniprot | |
| T1082 | Phosphorylation | Uniprot | |
| T1099 | O-Glycosylation | Uniprot | |
| T1106 | O-Glycosylation | Uniprot | |
| K1115 | Ubiquitination | Uniprot | |
| K1118 | Ubiquitination | Uniprot | |
| T1120 | O-Glycosylation | Uniprot | |
| T1124 | O-Glycosylation | Uniprot | |
| S1223 | Phosphorylation | Uniprot | |
| S1261 | Phosphorylation | Uniprot | |
| T1292 | Phosphorylation | Uniprot | |
| K1311 | Ubiquitination | Uniprot | |
| S1312 | Phosphorylation | Uniprot | |
| T1397 | Phosphorylation | Uniprot | |
| Y1419 | Phosphorylation | Uniprot | |
| S1433 | Phosphorylation | Uniprot | |
| S1438 | Phosphorylation | Uniprot | |
| Y1450 | Phosphorylation | Uniprot | |
| T1743 | Phosphorylation | Uniprot | |
| T1748 | O-Glycosylation | Uniprot | |
| T1749 | O-Glycosylation | Uniprot | |
| Y1897 | Phosphorylation | Uniprot | |
| T2062 | Phosphorylation | Uniprot | |
| S2125 | Phosphorylation | Uniprot | |
| Y2146 | Phosphorylation | Uniprot | |
| T2231 | Phosphorylation | Uniprot | |
| T2243 | Phosphorylation | Uniprot | |
| T2295 | O-Glycosylation | Uniprot | |
| T2299 | O-Glycosylation | Uniprot | |
| T2303 | O-Glycosylation | Uniprot | |
| T2309 | O-Glycosylation | Uniprot | |
| K2377 | Ubiquitination | Uniprot | |
| T2433 | Phosphorylation | Uniprot | |
| S2437 | Phosphorylation | Uniprot | |
| N2528 | N-Glycosylation | Uniprot | |
| S2536 | Phosphorylation | Uniprot | |
| S2541 | Phosphorylation | Uniprot | |
| S2591 | Phosphorylation | Uniprot | |
| K2605 | Ubiquitination | Uniprot | |
| K2675 | Ubiquitination | Uniprot | |
| N2679 | N-Glycosylation | Uniprot | |
| K2690 | Ubiquitination | Uniprot | |
| S2861 | Phosphorylation | Uniprot | |
| S2864 | Phosphorylation | Uniprot | |
| S2952 | Phosphorylation | Uniprot | |
| S2994 | Phosphorylation | Uniprot | |
| Y3043 | Phosphorylation | Uniprot | |
| S3062 | Phosphorylation | Uniprot |
研究背景
Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
The triple-helical tail is stabilized by disulfide bonds at each end.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Isoform 1 O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.
Secreted>Extracellular space>Extracellular matrix.
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM.
Trimer of identical chains each containing 190 kDa of non-triple-helical sequences.
Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
研究領域
· Organismal Systems > Digestive system > Protein digestion and absorption.
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