Phospho-ETS1 (Ser251) Antibody - #AF8371
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8371, RRID:AB_2840432.
折りたたみ／展開
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1; C ets 1 protein; c-ets-1; ETS 1; Ets protein; ETS proto-oncogene 1, transcription factor; ETS1; ETS1 oncogene; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; Oncogene ETS1; P54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E2 oncogene homolog 1; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1;
免疫原
Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
- P14921 ETS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEMMSQALKATFSGFTKEQQRLGIPKDPRQWTETHVRDWVMWAVNEFSLKGVDFQKFCMNGAALCALGKDCFLELAPDFVGDILWEHLEILQKEDVKPYQVNGVNPAYPESRYTSDYFISYGIEHAQCVPPSEFSEPSFITESYQTLHPISSEELLSLKYENDYPSVILRDPLQTDTLQNDYFAIKQEVVTPDNMCMGRTSRGKLGGQDSFESIESYDSCDRLTQSWSSQSSFNSLQRVPSYDSFDSEDYPAALPNHKPKGTFKDYVRDRADLNKDKPVIPAAALAGYTGSGPIQLWQFLLELLTDKSCQSFISWTGDGWEFKLSDPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQSLLGYTPEELHAMLDVKPDADE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14921 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Acetylation | Uniprot | |
| K2 | Ubiquitination | Uniprot | |
| K8 | Acetylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | P28482 (MAPK1) , Q13164 (MAPK7) , P27361 (MAPK3) | Uniprot |
| S41 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K138 | Sumoylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| K200 | Sumoylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| K245 | Acetylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S254 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| Y258 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| Y283 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S288 | Phosphorylation | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K305 | Acetylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K388 | Sumoylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| Y410 | Phosphorylation | Uniprot |
研究背景
Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
Cytoplasm. Nucleus.
Note: Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
Binds DNA as a homodimer; homodimerization is required for transcription activation. Interacts with MAF and MAFB. Interacts with PAX5; the interaction alters DNA-binding properties (By similarity). Interacts with DAXX. Interacts with UBE2I. Interacts with SP100; the interaction is direct and modulates ETS1 transcriptional activity.
Belongs to the ETS family.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
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