Phospho-IRS2 (Ser1100) Antibody - #AF8383

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製品: Phospho-IRS2 (Ser1100) Antibody
カタログ: AF8383
タンパク質の説明: Rabbit polyclonal antibody to Phospho-IRS2 (Ser1100)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Dog
分子量: 137kDa; 137kD(Calculated).
ユニプロット: Q9Y4H2
RRID: AB_2840444

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HPLC Report
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Dog(89%)
クローナリティ:
Polyclonal
特異性:
Phospho-IRS2 (Ser1100) Antibody detects endogenous levels of IRS2 only when phosphorylated at Ser1100.
RRID:
AB_2840444
引用形式: Affinity Biosciences Cat# AF8383, RRID:AB_2840444.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Insulin receptor substrate 2; IRS 2; IRS-2; Irs2; IRS2_HUMAN;

免疫原

免疫原:
Uniprot:

Q9Y4H2(IRS2_HUMAN) >>Visit HPA database.

遺伝子(ID):
IRS2(8660)
タンパク質配列:
MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTYASIDFLSHHLKEATIVKE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
89
Pig
67
Bovine
67
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4H2 基板として

Site PTM Type Enzyme
Phosphorylation
S3 Phosphorylation
S30 Phosphorylation
Y75 Phosphorylation
Y76 Phosphorylation
Y184 Phosphorylation
K295 Ubiquitination
S304 Phosphorylation
K305 Ubiquitination
S306 Phosphorylation P05771 (PRKCB)
S308 Phosphorylation
S309 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
T314 Phosphorylation
S334 Phosphorylation
S342 Phosphorylation
T344 Phosphorylation
S346 Phosphorylation
T350 Phosphorylation P45983 (MAPK8)
S358 Phosphorylation
T363 Phosphorylation
S365 Phosphorylation
S384 Phosphorylation
S388 Phosphorylation
S391 Phosphorylation P06493 (CDK1)
S400 Phosphorylation
T404 Phosphorylation
S406 Phosphorylation
R412 Methylation
R429 Methylation
S430 Phosphorylation
S432 Phosphorylation
S491 Phosphorylation P45983 (MAPK8)
S505 Phosphorylation
S506 Phosphorylation
S515 Phosphorylation
S518 Phosphorylation
T520 Phosphorylation
S523 Phosphorylation
T527 Phosphorylation
Y540 Phosphorylation
Y542 Phosphorylation
T544 Phosphorylation
S550 Phosphorylation
S560 Phosphorylation
R569 Methylation
T575 Phosphorylation
Y576 Phosphorylation
S577 Phosphorylation
T579 Phosphorylation
T580 Phosphorylation
S591 Phosphorylation
S592 Phosphorylation
S594 Phosphorylation
Y598 Phosphorylation
T599 Phosphorylation
T604 Phosphorylation
S606 Phosphorylation
S608 Phosphorylation
S615 Phosphorylation
S619 Phosphorylation
S620 Phosphorylation
Y625 Phosphorylation
Y628 Phosphorylation P06213 (INSR)
Y632 Phosphorylation P06213 (INSR)
S639 Phosphorylation
S642 Phosphorylation
S645 Phosphorylation
Y653 Phosphorylation
T657 Phosphorylation P28482 (MAPK1)
S672 Phosphorylation
Y675 Phosphorylation
S679 Phosphorylation P05771 (PRKCB)
S682 Phosphorylation
T713 Phosphorylation
S714 Phosphorylation
S723 Phosphorylation
S730 Phosphorylation
S731 Phosphorylation
S735 Phosphorylation
S736 Phosphorylation
S740 Phosphorylation
Y742 Phosphorylation
S752 Phosphorylation
Y766 Phosphorylation
S770 Phosphorylation
S772 Phosphorylation
T776 Phosphorylation
T777 Phosphorylation
T779 Phosphorylation
Y803 Phosphorylation
S804 Phosphorylation
S805 Phosphorylation
S809 Phosphorylation
Y810 Phosphorylation
Y814 Phosphorylation
T815 Phosphorylation
S820 Phosphorylation
Y823 Phosphorylation
S827 Phosphorylation
S828 Phosphorylation
T891 Phosphorylation
S894 Phosphorylation
S903 Phosphorylation
S915 Phosphorylation
Y919 Phosphorylation
S932 Phosphorylation
S973 Phosphorylation
S976 Phosphorylation
Y978 Phosphorylation
S985 Phosphorylation
T1082 Phosphorylation
S1100 Phosphorylation
S1103 Phosphorylation
S1109 Phosphorylation
K1134 Methylation
S1148 Phosphorylation
S1149 Phosphorylation
T1151 Phosphorylation
S1153 Phosphorylation
S1154 Phosphorylation
T1156 Phosphorylation
T1157 Phosphorylation
T1159 Phosphorylation
S1162 Phosphorylation
S1164 Phosphorylation
K1170 Methylation
S1174 Phosphorylation
S1176 Phosphorylation
S1181 Phosphorylation
S1185 Phosphorylation
S1186 Phosphorylation
T1202 Phosphorylation
S1203 Phosphorylation
T1221 Phosphorylation
S1234 Phosphorylation
S1237 Phosphorylation
T1243 Phosphorylation
Y1253 Phosphorylation
S1283 Phosphorylation
Y1320 Phosphorylation

研究背景

機能:

May mediate the control of various cellular processes by insulin.

細胞の位置付け:

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with PHIP.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

参考文献

1). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences, 2023 (PubMed: 38146533) [IF=5.0]

Application: WB    Species: Human    Sample: adipose tissue

FIGURE 11 Involvement of SHBG in the insulin signaling pathway. Relative expression levels of PI3K (A), AKT1 (B), GLUT4 (C), IRS1 (D), IRS2 (E), INSR (F) were analyzed by qRT-PCR. Protein levels of PI3K (G), AKT (H), GLUT4 (I), IRS1 (J), IRS2 (K), SHBG (L), IR (M) were analyzed by Western Blot. Representative immunoblots (N). Representative data are shown as mean ± SD.
2). Chronic timed sleep restriction attenuates LepRb-mediated signaling pathways and circadian clock gene expression in the rat hypothalamus. Frontiers in Neuroscience, 2020 (PubMed: 33013300) [IF=4.3]

Application: WB    Species: rat    Sample:

FIGURE 4 | Sleep restriction induced depletion of circadian clock expression. (A) Western blot analysis. Images are representative of five independent experiments for each group.

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