Phospho-RBL2 (Ser639) Antibody - #AF8401
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8401, RRID:AB_2840462.
折りたたみ／展開
130 kDa retinoblastoma associated protein; 130 kDa retinoblastoma-associated protein; IB4; p130; PPAR-alpha-interacting complex protein 128; PRB2; Rb2; Rbl2; RBL2_HUMAN; RBR 2; RBR-2; RBR2; Retinoblastoma like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2; RIC128;
免疫原
- Q08999 RBL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFFRKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q08999 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S98 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| T401 | Phosphorylation | P11802 (CDK4) , Q00534 (CDK6) | Uniprot |
| T404 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T417 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K443 | Ubiquitination | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| Y544 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T642 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T647 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
| T661 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T664 | Phosphorylation | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| Y667 | Phosphorylation | Uniprot | |
| Y670 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Q00534 (CDK6) , P11802 (CDK4) | Uniprot |
| S676 | Phosphorylation | Uniprot | |
| T678 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S690 | Phosphorylation | Uniprot | |
| T694 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S941 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S952 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T954 | Phosphorylation | Uniprot | |
| T961 | Phosphorylation | Uniprot | |
| S962 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| S973 | Phosphorylation | Uniprot | |
| T974 | Phosphorylation | Uniprot | |
| S981 | Phosphorylation | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| T986 | Phosphorylation | Uniprot | |
| T988 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| T1019 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | P11802 (CDK4) , Q00534 (CDK6) | Uniprot |
| Y1037 | Phosphorylation | Uniprot | |
| T1042 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | P24941 (CDK2) | Uniprot |
| Y1057 | Phosphorylation | Uniprot | |
| S1059 | Phosphorylation | Uniprot | |
| T1065 | Phosphorylation | Uniprot | |
| S1068 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1078 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1082 | Phosphorylation | Uniprot | |
| S1090 | Phosphorylation | Uniprot | |
| T1097 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1112 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1138 | Phosphorylation | Uniprot |
研究背景
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
Nucleus.
Interacts with AATF. Interacts with KMT5B, KMT5C and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with RBBP9 (By similarity).
Belongs to the retinoblastoma protein (RB) family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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