Phospho-IRS1 (Ser307) Antibody - #AF3272
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3272, RRID:AB_2834329.
折りたたみ/展開
HIRS 1; HIRS1; Insulin receptor substrate 1; IRS 1; IRS-1; IRS1; IRS1_HUMAN; OTTHUMP00000164234;
免疫原
- P35568 IRS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRSKSQSSSNCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSARLHPPLNHSRSIPMPASRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGPSTLTAPNGHYILSRGGNGHRCTPGTGLGTSPALAGDEAASAADLDNRFRKRTHSAGTSPTITHQKTPSQSSVASIEEYTEMMPAYPPGGGSGGRLPGHRHSAFVPTRSYPEEGLEMHPLERRGGHHRPDSSTLHTDDGYMPMSPGVAPVPSGRKGSGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGGCSPDIGGGPSSSSSSSNAVPSGTSYGKLWTNGVGGHHSHVLPHPKPPVESSGGKLLPCTGDYMNMSPVGDSNTSSPSDCYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSTSSGRLLYAATADDSSSSTSSDSLGGGYCGARLEPSLPHPHHQVLQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRAREQQQQQQPLLHPPEPKSPGEYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35568 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Phosphorylation | Uniprot | ||
S3 | Phosphorylation | Uniprot | |
S24 | Phosphorylation | P17252 (PRKCA) , P53350 (PLK1) , P67870 (CSNK2B) | Uniprot |
Y46 | Phosphorylation | Uniprot | |
Y47 | Phosphorylation | Uniprot | |
K52 | Acetylation | Uniprot | |
K61 | Acetylation | Uniprot | |
Y87 | Phosphorylation | Uniprot | |
T88 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
S99 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
K169 | Ubiquitination | Uniprot | |
Y183 | Phosphorylation | Uniprot | |
S268 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S270 | Phosphorylation | Q13535 (ATR) , P23443 (RPS6KB1) , O14920 (IKBKB) | Uniprot |
S272 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S273 | Phosphorylation | Uniprot | |
S274 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S303 | Phosphorylation | Uniprot | |
T305 | Phosphorylation | Uniprot | |
S307 | Phosphorylation | Q02750 (MAP2K1) , Q05655 (PRKCD) , P45984 (MAPK9) , P23443 (RPS6KB1) , O14920 (IKBKB) , P45983 (MAPK8) , P42345 (MTOR) | Uniprot |
T309 | Phosphorylation | Uniprot | |
T311 | Phosphorylation | Uniprot | |
S312 | Phosphorylation | P28482 (MAPK1) , O14733 (MAP2K7) , O14920 (IKBKB) , P45983 (MAPK8) | Uniprot |
S315 | Phosphorylation | P45983 (MAPK8) , P45984 (MAPK9) | Uniprot |
S323 | Phosphorylation | Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ) | Uniprot |
S329 | Phosphorylation | Uniprot | |
S330 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
T335 | Phosphorylation | Uniprot | |
S337 | Phosphorylation | Uniprot | |
S341 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S345 | Phosphorylation | O14920 (IKBKB) | Uniprot |
S348 | Phosphorylation | Uniprot | |
S350 | Phosphorylation | Uniprot | |
T351 | Phosphorylation | Uniprot | |
S362 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
S372 | Phosphorylation | Uniprot | |
R373 | Methylation | Uniprot | |
S374 | Phosphorylation | Uniprot | |
S383 | Phosphorylation | Uniprot | |
S385 | Phosphorylation | Uniprot | |
S388 | Phosphorylation | Uniprot | |
S391 | Phosphorylation | Uniprot | |
S417 | Phosphorylation | Uniprot | |
S419 | Phosphorylation | Uniprot | |
Y431 | Phosphorylation | Uniprot | |
S441 | Phosphorylation | Q05655 (PRKCD) , P05771 (PRKCB) , Q05513 (PRKCZ) | Uniprot |
S444 | Phosphorylation | Uniprot | |
T446 | Phosphorylation | Uniprot | |
S449 | Phosphorylation | Uniprot | |
T453 | Phosphorylation | Uniprot | |
S463 | Phosphorylation | Uniprot | |
Y465 | Phosphorylation | P08069 (IGF1R) | Uniprot |
Y483 | Phosphorylation | Uniprot | |
S486 | Phosphorylation | Uniprot | |
T495 | Phosphorylation | Uniprot | |
T498 | Phosphorylation | Uniprot | |
T502 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
S503 | Phosphorylation | Uniprot | |
T525 | Phosphorylation | Uniprot | |
S527 | Phosphorylation | P23443 (RPS6KB1) , O14920 (IKBKB) | Uniprot |
T530 | Phosphorylation | Uniprot | |
S531 | Phosphorylation | O14920 (IKBKB) | Uniprot |
T533 | Phosphorylation | Uniprot | |
S574 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
S581 | Phosphorylation | Uniprot | |
S603 | Phosphorylation | Uniprot | |
S604 | Phosphorylation | Uniprot | |
T605 | Phosphorylation | Uniprot | |
T608 | Phosphorylation | Uniprot | |
Y612 | Phosphorylation | P23458 (JAK1) , P08069 (IGF1R) , P45984 (MAPK9) | Uniprot |
S616 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8) , Q05655 (PRKCD) | Uniprot |
S624 | Phosphorylation | Uniprot | |
S629 | Phosphorylation | P31749 (AKT1) | Uniprot |
Y632 | Phosphorylation | P45984 (MAPK9) , P23458 (JAK1) , P08069 (IGF1R) | Uniprot |
S636 | Phosphorylation | P23443 (RPS6KB1) , O75116 (ROCK2) , P45983 (MAPK8) , P28482 (MAPK1) | Uniprot |
S639 | Phosphorylation | P28482 (MAPK1) | Uniprot |
Y662 | Phosphorylation | P08069 (IGF1R) , P23458 (JAK1) | Uniprot |
S666 | Phosphorylation | Uniprot | |
S668 | Phosphorylation | Uniprot | |
S672 | Phosphorylation | Uniprot | |
S680 | Phosphorylation | Uniprot | |
S694 | Phosphorylation | Uniprot | |
Y695 | Phosphorylation | Uniprot | |
Y732 | Phosphorylation | P23458 (JAK1) , P08069 (IGF1R) | Uniprot |
S736 | Phosphorylation | Uniprot | |
S766 | Phosphorylation | Uniprot | |
K772 | Methylation | Uniprot | |
K772 | Ubiquitination | Uniprot | |
S794 | Phosphorylation | Q9H0K1 (SIK2) | Uniprot |
T811 | Phosphorylation | P67870 (CSNK2B) | Uniprot |
T847 | Phosphorylation | Uniprot | |
T859 | Phosphorylation | Uniprot | |
S862 | Phosphorylation | Uniprot | |
K867 | Ubiquitination | Uniprot | |
S892 | Phosphorylation | Uniprot | |
Y896 | Phosphorylation | P06241 (FYN) , P08069 (IGF1R) , P06213 (INSR) | Uniprot |
T936 | Phosphorylation | Uniprot | |
T938 | Phosphorylation | Uniprot | |
Y941 | Phosphorylation | P08069 (IGF1R) | Uniprot |
S974 | Phosphorylation | O75116 (ROCK2) | Uniprot |
S984 | O-Glycosylation | Uniprot | |
S984 | Phosphorylation | Uniprot | |
S985 | O-Glycosylation | Uniprot | |
Y989 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) | Uniprot |
S1005 | Phosphorylation | Uniprot | |
S1011 | O-Glycosylation | Uniprot | |
Y1012 | Phosphorylation | Uniprot | |
S1025 | Phosphorylation | Uniprot | |
S1037 | Phosphorylation | Uniprot | |
S1041 | Phosphorylation | Uniprot | |
S1043 | Phosphorylation | Uniprot | |
T1045 | Phosphorylation | Uniprot | |
S1078 | Phosphorylation | Uniprot | |
S1084 | Phosphorylation | Uniprot | |
S1100 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S1101 | Phosphorylation | P05129 (PRKCG) , Q05513 (PRKCZ) , Q04759 (PRKCQ) , Q05655 (PRKCD) , P23443 (RPS6KB1) | Uniprot |
T1103 | Phosphorylation | Uniprot | |
S1105 | Phosphorylation | Uniprot | |
T1107 | Phosphorylation | Uniprot | |
S1109 | Phosphorylation | Uniprot | |
T1111 | Phosphorylation | Uniprot | |
T1116 | Phosphorylation | Uniprot | |
S1142 | Phosphorylation | Uniprot | |
S1143 | Phosphorylation | Uniprot | |
S1145 | Phosphorylation | Uniprot | |
Y1179 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) , P06241 (FYN) | Uniprot |
S1222 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S1223 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y1229 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) | Uniprot |
研究背景
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity). Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1.
Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
Interacts with UBTF and PIK3CA (By similarity). Interacts (via phosphorylated YXXM motifs) with PIK3R1 (By similarity). Interacts with ROCK1 and FER (By similarity). Interacts (via PH domain) with PHIP (By similarity). Interacts with GRB2 (By similarity). Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Interacts with EIF2AK2/PKR (By similarity). Interacts with GKAP1 (By similarity). Interacts with DGKZ in the absence of insulin; insulin stimulation decreases this interaction (By similarity). Found in a ternary complex with DGKZ and PIP5K1A in the absence of insulin stimulation (By similarity).
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
参考文献
Application: WB Species: rat Sample: liver
Application: WB Species: Mice Sample: epididymal white adipose tissue
Application: WB Species: mouse Sample: Liver
Application: WB Species: Human Sample: HepG2 cells
Application: WB Species: mouse Sample: placenta
Application: WB Species: Mice Sample: serum and placenta tissue
Application: WB Species: human Sample: HepG2 cells
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