Phospho-ROS (Tyr2114) Antibody - #AF8452

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製品: Phospho-ROS (Tyr2114) Antibody
カタログ: AF8452
タンパク質の説明: Rabbit polyclonal antibody to Phospho-ROS (Tyr2114)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
分子量: 263KD; 264kD(Calculated).
ユニプロット: P08922
RRID: AB_2840507

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HPLC Report
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-ROS (Tyr2114) Antibody detects endogenous levels of ROS only when phosphorylated at Tyr2114.
RRID:
AB_2840507
引用形式: Affinity Biosciences Cat# AF8452, RRID:AB_2840507.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

c ros 1; c ros; c ros oncogene 1 receptor tyrosine kinase; c Ros receptor tyrosine kinase; c ros1; MCF 3; MCF3; Oncogene ROS; OTTHUMP00000017814; OTTHUMP00000017815; OTTHUMP00000040389; Proto oncogene c ros 1 protein; Proto oncogene c Ros; Proto oncogene tyrosine protein kinase ROS; Proto oncogene tyrosine protein kinase ROS precursor; Proto-oncogene c-Ros-1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c ros oncogene 1; Ros 1; ROS 1C; ROS; ROS proto oncogene 1 , receptor tyrosine kinase; ROS_HUMAN; ROS1; ROS1C; Transmembrane tyrosine specific protein kinase; v ros avian UR2 sarcoma virus oncogene homolog 1; v ros UR2 sarcoma virus oncogene homolog 1;

免疫原

免疫原:
Uniprot:

P08922(ROS1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ROS1(6098)
発現特異性:
P08922 ROS1_HUMAN:

Expressed in brain. Expression is increased in primary gliomas.

タンパク質配列:
MKNIYCLIPKLVNFATLGCLWISVVQCTVLNSCLKSCVTNLGQQLDLGTPHNLSEPCIQGCHFWNSVDQKNCALKCRESCEVGCSSAEGAYEEEVLENADLPTAPFASSIGSHNMTLRWKSANFSGVKYIIQWKYAQLLGSWTYTKTVSRPSYVVKPLHPFTEYIFRVVWIFTAQLQLYSPPSPSYRTHPHGVPETAPLIRNIESSSPDTVEVSWDPPQFPGGPILGYNLRLISKNQKLDAGTQRTSFQFYSTLPNTIYRFSIAAVNEVGEGPEAESSITTSSSAVQQEEQWLFLSRKTSLRKRSLKHLVDEAHCLRLDAIYHNITGISVDVHQQIVYFSEGTLIWAKKAANMSDVSDLRIFYRGSGLISSISIDWLYQRMYFIMDELVCVCDLENCSNIEEITPPSISAPQKIVADSYNGYVFYLLRDGIYRADLPVPSGRCAEAVRIVESCTLKDFAIKPQAKRIIYFNDTAQVFMSTFLDGSASHLILPRIPFADVKSFACENNDFLVTDGKVIFQQDALSFNEFIVGCDLSHIEEFGFGNLVIFGSSSQLHPLPGRPQELSVLFGSHQALVQWKPPALAIGANVILISDIIELFELGPSAWQNWTYEVKVSTQDPPEVTHIFLNISGTMLNVPELQSAMKYKVSVRASSPKRPGPWSEPSVGTTLVPASEPPFIMAVKEDGLWSKPLNSFGPGEFLSSDIGNVSDMDWYNNSLYYSDTKGDVFVWLLNGTDISENYHLPSIAGAGALAFEWLGHFLYWAGKTYVIQRQSVLTGHTDIVTHVKLLVNDMVVDSVGGYLYWTTLYSVESTRLNGESSLVLQTQPWFSGKKVIALTLDLSDGLLYWLVQDSQCIHLYTAVLRGQSTGDTTITEFAAWSTSEISQNALMYYSGRLFWINGFRIITTQEIGQKTSVSVLEPARFNQFTIIQTSLKPLPGNFSFTPKVIPDSVQESSFRIEGNASSFQILWNGPPAVDWGVVFYSVEFSAHSKFLASEQHSLPVFTVEGLEPYALFNLSVTPYTYWGKGPKTSLSLRAPETVPSAPENPRIFILPSGKCCNKNEVVVEFRWNKPKHENGVLTKFEIFYNISNQSITNKTCEDWIAVNVTPSVMSFQLEGMSPRCFIAFQVRAFTSKGPGPYADVVKSTTSEINPFPHLITLLGNKIVFLDMDQNQVVWTFSAERVISAVCYTADNEMGYYAEGDSLFLLHLHNRSSSELFQDSLVFDITVITIDWISRHLYFALKESQNGMQVFDVDLEHKVKYPREVKIHNRNSTIISFSVYPLLSRLYWTEVSNFGYQMFYYSIISHTLHRILQPTATNQQNKRNQCSCNVTEFELSGAMAIDTSNLEKPLIYFAKAQEIWAMDLEGCQCWRVITVPAMLAGKTLVSLTVDGDLIYWIITAKDSTQIYQAKKGNGAIVSQVKALRSRHILAYSSVMQPFPDKAFLSLASDTVEPTILNATNTSLTIRLPLAKTNLTWYGITSPTPTYLVYYAEVNDRKNSSDLKYRILEFQDSIALIEDLQPFSTYMIQIAVKNYYSDPLEHLPPGKEIWGKTKNGVPEAVQLINTTVRSDTSLIISWRESHKPNGPKESVRYQLAISHLALIPETPLRQSEFPNGRLTLLVTRLSGGNIYVLKVLACHSEEMWCTESHPVTVEMFNTPEKPYSLVPENTSLQFNWKAPLNVNLIRFWVELQKWKYNEFYHVKTSCSQGPAYVCNITNLQPYTSYNVRVVVVYKTGENSTSLPESFKTKAGVPNKPGIPKLLEGSKNSIQWEKAEDNGCRITYYILEIRKSTSNNLQNQNLRWKMTFNGSCSSVCTWKSKNLKGIFQFRVVAANNLGFGEYSGISENIILVGDDFWIPETSFILTIIVGIFLVVTIPLTFVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEKQVAYCPSGKPEGLNYACLTHSGYGDGSD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Zebrafish
88
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08922 基板として

Site PTM Type Enzyme
Y135 Phosphorylation
T143 Phosphorylation
Y144 Phosphorylation
T145 Phosphorylation
T188 Phosphorylation
T196 Phosphorylation
Y800 Phosphorylation
Y802 Phosphorylation
Y807 Phosphorylation
S818 Phosphorylation
S1185 Phosphorylation
T1190 Phosphorylation
K1259 Acetylation
S1273 Phosphorylation
T1274 Phosphorylation
T1375 Phosphorylation
K1504 Acetylation
K1749 Acetylation
Y1923 Phosphorylation
T1927 Phosphorylation
T1930 Phosphorylation
K1944 Acetylation
S2002 Phosphorylation
Y2023 Phosphorylation
Y2069 Phosphorylation
Y2110 Phosphorylation
Y2114 Phosphorylation
Y2115 Phosphorylation
Y2173 Phosphorylation
Y2227 Phosphorylation
Y2274 Phosphorylation P08922 (ROS1)
Y2323 Phosphorylation
S2326 Phosphorylation
Y2334 Phosphorylation P08922 (ROS1)
T2338 Phosphorylation
Y2342 Phosphorylation
S2346 Phosphorylation

PTMs - P08922 酵素として

Substrate Site Source
P08922 (ROS1) Y2274 Uniprot
P08922 (ROS1) Y2334 Uniprot
Q06124 (PTPN11) Y546 Uniprot
Q06124 (PTPN11) Y584 Uniprot

研究背景

機能:

Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.

PTMs:

Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in brain. Expression is increased in primary gliomas.

サブユニット構造:

Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation (By similarity). Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway. Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

参考文献

1). Tanshinone I Inhibits Oxidative Stress–Induced Cardiomyocyte Injury by Modulating Nrf2 Signaling. Frontiers in Pharmacology, 2021 (PubMed: 34084132) [IF=5.6]

Application: IHC    Species: Mouse    Sample: Heart tissue

FIGURE 5 Tan I inhibits ISO-induced myocardial injury in a mouse model. Intraperitoneal injection of ISO was performed to establish an oxidative stress–induced myocardial damage model. (A) Hematoxylin–eosin (HE) staining; Masson’s trichrome staining; and immunohistochemistry (IHC) analysis, including p-ROS, BAX, caspase3, and Bcl-2; TUNEL analysis; and transthoracic echocardiography analysis. (B) Relative expression of p-ROS. (C) Heart weight/body weight ratio. (D) Left ventricular ejection fraction (LVEF). (E) Left ventricular fractional shortening (LVFS). (F) Left ventricular end-systolic dimension (LVESD). (G) Left ventricular end-diastolic dimension (LVEDD). (H) Collagen volume fraction. (I) Apoptotic index. (J) Relative expression of BAX. (K) Relative expression of caspase3. (L) Relative expression of Bcl-2. Data are presented as mean ± S.D. # p < 0.05, ## p < 0.01, compared to the sham group. *p < 0.05, **p < 0.01, compared to the model group (n = 8).

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